Click image to see more details
Product Info Summary
| SKU: | M02431-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-14-3-3 YWHAB Rabbit Monoclonal Antibody
SKU/Catalog Number
M02431-2
BM4758 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-14-3-3 YWHAB Rabbit Monoclonal Antibody catalog # M02431-2. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-14-3-3 YWHAB Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02431-2)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
HHG-25
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human 41701
Reactive Species
M02431-2 is reactive to YWHAB in Human, Mouse, Rat
Observed Molecular Weight
28 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02431-2 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
Positive Control
WB: HeLa cell, 3T3 cell, PC-12 cell
Validation Images & Assay Conditions
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Western blot analysis of 14-3-3 expression in (1) HeLa cell lysate; (2) 3T3 cell lysate; (3) PC-12 cell lysate.
Specific Publications For Anti-14-3-3 YWHAB Rabbit Monoclonal Antibody (M02431-2)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-14-3-3 YWHAB Rabbit Monoclonal Antibody
Question
Will M02431-2 anti-14-3-3 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
It shows on the product datasheet, M02431-2 anti-14-3-3 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-25
Question
I was wanting to use your anti-14-3-3 Rabbit Monoclonal antibody for WB for human keratinocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human keratinocyte identification?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
You can see on the product datasheet, M02431-2 anti-14-3-3 Rabbit Monoclonal antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human keratinocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-03
Question
I would like to test anti-14-3-3 Rabbit Monoclonal antibody M02431-2 on human keratinocyte for research purposes, then I may be interested in using anti-14-3-3 Rabbit Monoclonal antibody M02431-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
The products we sell, including anti-14-3-3 Rabbit Monoclonal antibody M02431-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-26
Question
I see that the anti-14-3-3 Rabbit Monoclonal antibody M02431-2 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-06-06
Question
Does anti-14-3-3 Rabbit Monoclonal antibody M02431-2 work on feline WB with thymus?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
Our lab technicians have not tested anti-14-3-3 Rabbit Monoclonal antibody M02431-2 on feline. You can run a BLAST between feline and the immunogen sequence of anti-14-3-3 Rabbit Monoclonal antibody M02431-2 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline thymus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-21
Question
Is this M02431-2 anti-14-3-3 Rabbit Monoclonal antibody reactive to the isotypes of YWHAB?
Verified Customer
Verified customer
Asked: 2019-03-04
Answer
The immunogen of M02431-2 anti-14-3-3 Rabbit Monoclonal antibody is A synthesized peptide derived from human 41701. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-03-04
Question
Is there a BSA free version of anti-14-3-3 Rabbit Monoclonal antibody M02431-2 available?
Verified Customer
Verified customer
Asked: 2019-01-24
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-14-3-3 Rabbit Monoclonal antibody M02431-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-01-24
