Anti-1433 Eta (eta) Monoclonal Antibody

Product Name

Anti-1433 Eta (eta) Monoclonal Antibody

View all 14-3-3 eta Antibodies

SKU/Catalog Number

M04219

Size

100ul

Description

Boster Bio Anti-1433 Eta (eta) Monoclonal Antibody catalog # M04219. Tested in IF, IHC, WB applications. This antibody reacts with Bovine, Canine, Equine, Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-1433 Eta (eta) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04219)

Host

Mouse

Contents

Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN₃

Clonality

Monoclonal

Immunogen

Full length human recombinant 14.3.3 (eta) protein expressed in and purified from E.coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M04219 is reactive to YWHAH in Bovine, Canine, Equine, Human, Mouse, Rat

Applications

M04219 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

28.219kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:5,000. IF/ICC and IHC: 1:1,000.

Validation Images & Assay Conditions

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Western blot analysis of whole brain lysates (lanes 2,3), and cell lysates (lanes 4-8), using mouse mAb to 14.3.3, M04219, dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y, [8] C6 cells. Strong band at 28kDa corresponds to 14.3.3 protein, expressed in all preparations.

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Immunofluorescent analysis of HeLa cells stained with mouse mAb to 14.3.3, M04219, dilution 1:1,000 in red. Blue is DAPI staining of nuclear DNA. The M04219 antibody reveals the diffuse cytoplasmic distribution of 14.3.3 protein with higher concentration in the perinuclear region.

Gene/Protein Information For YWHAH (Source: Uniprot.org, NCBI)

Alternative Names

1433 eta; 14-3-3 eta; 14-3-3 protein eta; Protein AS1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, etapolypeptide; YWHA1; YWHA114-3-3 eta; YWHAH YWHAH YWHA1 tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein eta 14-3-3 protein eta|14-3-3 eta|tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide

For more info on YWHAH, check out the YWHAH Infographic

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In this infographic, you will see the following information for YWHAH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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