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Product Info Summary
| SKU: | A01593-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-5-HT2C Receptor/HTR2C Antibody Picoband®
SKU/Catalog Number
A01593-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-5-HT2C Receptor/HTR2C Antibody Picoband® catalog # A01593-1. Tested in ELISA, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-5-HT2C Receptor/HTR2C Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01593-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human 5-HT2C Receptor/HTR2C recombinant protein (Position: N372-V458).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01593-1 is reactive to HTR2C in Human, Rat
Observed Molecular Weight
75 kDa
Calculated molecular weight
51.8 kDa
Background of HTR2C
This gene encodes a seven-transmembrane G-protein-coupled receptor. The encoded protein responds to signaling through the neurotransmitter serotonin. The mRNA of this gene is subject to multiple RNA editing events, where adenosine residues encoded by the genome are converted to inosines. RNA editing is predicted to alter the structure of the second intracellular loop, thereby generating alternate protein forms with decreased ability to interact with G proteins. Abnormalities in RNA editing of this gene have been detected in victims of suicide that suffer from depression. In addition, naturally-occuring variation in the promoter and 5' non-coding and coding regions of this gene may show statistically-significant association with mental illness and behavioral disorders. Alternative splicing results in multiple different transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01593-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat brain tissue, rat C6 whole cell
Validation Images & Assay Conditions
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Western blot analysis of 5-HT2C Receptor/HTR2C using anti-5-HT2C Receptor/HTR2C antibody (A01593-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat C6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-5-HT2C Receptor/HTR2C antigen affinity purified polyclonal antibody (Catalog # A01593-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 5-HT2C Receptor/HTR2C at approximately 75 kDa. The expected band size for 5-HT2C Receptor/HTR2C is at 52 kDa.
Specific Publications For Anti-5-HT2C Receptor/HTR2C Antibody Picoband® (A01593-1)
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