Anti-ABCB5 Antibody

Product Name

Anti-ABCB5 Antibody

View all ABCB5 Antibodies

SKU/Catalog Number

A02979

Size

100ug

Form

Liquid (sterile filtered)

Description

ABC transporter (ABCB5) implicated in drug efflux/chemoresistance and melanoma cell subsets per product background; rhodamine efflux transporter; research use in cancer and signal transduction. Assay: rabbit polyclonal, WB/IHC/ELISA; observed ~42 kDa band (page), calculated ~138.6 kDa. Often profiled with growth/neuronal factors like BDNF (putative, tumor–nerve crosstalk) or metabolic markers such as FABP2/I-FABP when studying tumor metabolism (putative).

Storage & Handling

Store Anti-ABCB5 at -20°C prior to opening. Aliquot antibody and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-ABCB5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02979)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

ABCB5 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues corresponding to an internal region of human ABCB5.

Reactive Species

A02979 is reactive to ABCB5 in Human

Observed Molecular Weight

42 kDa

Calculated molecular weight

138.6 kDa

Background of ABCB5

This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Anti-ABCB5 Antibody is a ATP-Binding Cassette protein. ABCB5 is a novel member of the human P-glycoprotein family. It functions as a determinant of membrane potential and regulator of cell fusion in physiologic skin progenitor cells. Cell fusion is thought to contribute to tissue differentiation. ABCB5 is predominantly expressed in melanoma cells and is a novel molecular marker for a distinct subset of chemoresistant stem cell phenotype-expressing tumor cells among human epidermal melanocytes. ABCB5 is a rhodamine efflux transporter. This antibody product is intended to be used to confirm cellular localization and expression level of ABCB5. ABCB5 antibody is ideal for Cancer and Signal Transduction research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A02979 is guaranteed for ELISA, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:50,000
IHC: 1:200
WB: 1:20,000
This affinity purified ABCB5 antibody has been tested for use in ELISA, IHC, and western blotting.  Specific conditions for reactivity should be optimized by the end user.

Validation Images & Assay Conditions

See full target information ABCB5

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