Anti-ABCB8 Antibody

ABCB8 antibody

Boster Bio Anti-ABCB8 Antibody (Catalog # A08344). Tested in ELISA, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A08344
Size: 100uL
Reactive Species: Human
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-ABCB8 Antibody

View all ABCB8 Antibodies

SKU/Catalog Number

A08344

Size

100uL

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-ABCB8 Antibody (Catalog # A08344). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-ABCB8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08344)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

ABCB8 was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a internal portion of human ABCB8.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A08344 is reactive to ABCB8 in Human

Applications

A08344 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

79.989kDa

Background of ABCB8

This antibody is suitable for Cancer Research and drug resistance. It has been found that ABC transporters are involved in mulitdrug resistance through their ability to control the presence of drugs in cells. Multidrug resistance is currently being looked at in particular reference to cancer that is resistant to treatment. ABCB8 seems to have particularly high resistance rates in renal cancer and cancer of the central nervous system. Another significant aspect of ABCB8 is that it is a mitochondrial transporter. There are very few ABC transporters localized to the mitochondria which implies information about them could be extremely important. ABCB8 may also be localized to other parts of the cell which are yet to be determined. Anti-ABCB8 antibody is ideal for researchers interested in Cancer Research and drug resistance.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:20,000 - 1:50,000
WB: 1:250-1:500

Validation Images & Assay Conditions

Gene/Protein Information For ABCB8 (Source: Uniprot.org, NCBI)

Gene Name

ABCB8

Full Name

Mitochondrial potassium channel ATP-binding subunit

Weight

79.989kDa

Superfamily

ABC transporter superfamily

Alternative Names

ATP-binding cassette, sub-family B (MDR/TAP), member 8; EC 3.6.3; EST328128; M-ABC1mitochondrial ABC protein; Mitochondrial ATP-binding cassette 1; mitochondrial ABCB8 EST328128, M-ABC1, MABC1, MITOSUR ATP binding cassette subfamily B member 8 mitochondrial potassium channel ATP-binding subunit|ATP-binding cassette sub-family B member 8, mitochondrial|ATP-binding cassette, sub-family B (MDR/TAP), member 8|mitochondrial ABC protein|mitochondrial ATP-binding cassette 1|mitochondrial sulfonylurea-receptor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ABCB8, check out the ABCB8 Infographic

ABCB8 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ABCB8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-ABCB8 Antibody

Question

Here is the WB image, lot number and protocol we used for kidney using anti-ABCB8 antibody A08344. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-02-13

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-13

Question

We are currently using anti-ABCB8 antibody A08344 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-10-16

Answer

The anti-ABCB8 antibody (A08344) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-10-16

Question

Will A08344 anti-ABCB8 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-09-20

Answer

It shows on the product datasheet, A08344 anti-ABCB8 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-09-20

Question

Will anti-ABCB8 antibody A08344 work for WB with kidney?

Verified Customer

Verified customer

Asked: 2019-05-15

Answer

According to the expression profile of kidney, ABCB8 is highly expressed in kidney. So, it is likely that anti-ABCB8 antibody A08344 will work for WB with kidney.

Boster Scientific Support

Answered: 2019-05-15

Question

Is a blocking peptide available for product anti-ABCB8 antibody (A08344)?

T. Edwards

Verified customer

Asked: 2016-01-21

Answer

We do provide the blocking peptide for product anti-ABCB8 antibody (A08344). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2016-01-21

Question

Will anti-ABCB8 antibody A08344 work on feline WB with left adrenal gland?

A. Edwards

Verified customer

Asked: 2014-08-07

Answer

Our lab technicians have not validated anti-ABCB8 antibody A08344 on feline. You can run a BLAST between feline and the immunogen sequence of anti-ABCB8 antibody A08344 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline left adrenal gland in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2014-08-07

Size

Total: $599

SKU:A08344

Backordered.

Lead time for this item is typically 4-6 days

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A08344
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$599.00

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