Anti-ABCC3 Antibody Picoband™

Product Name

Anti-ABCC3 Antibody Picoband™

View all MRP3 Antibodies

SKU/Catalog Number

A02429-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-ABCC3 Antibody Picoband™ catalog # A02429-2. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-ABCC3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02429-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human ABCC3 recombinant protein (Position: H22-G1351).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02429-2 is reactive to ABCC3 in Human

Applications

A02429-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

200 kDa

Calculated molecular weight

169.343kDa

Background of MRP3

Canalicular multispecific organic anion transporter 2 is a protein that in humans is encoded by the ABCC3 gene. The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. The specific function of this protein has not yet been determined; however, this protein may play a role in the transport of biliary and intestinal excretion of organic anions. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg /1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of ABCC3 using anti-ABCC3 antibody (A02429-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCC3 antigen affinity purified polyclonal antibody (Catalog # A02429-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCC3 at approximately 200 kDa. The expected band size for ABCC3 is at 200 kDa.

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Figure 2. Flow Cytometry analysis of HepG2 cells using anti-ABCC3 antibody (A02429-2).
Overlay histogram showing HepG2 cells stained with A02429-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ABCC3 Antibody (A02429-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For ABCC3 (Source: Uniprot.org, NCBI)

Alternative Names

ABC31; ATP-binding cassette sub-family C member 3; ATP-binding cassette, sub-family C (CFTR/MRP), member 3; canalicular multispecific organic anion transporter 2; canicular multispecific organic anion transporter; cMOAT2; EC 3.6.3; EC 3.6.3.44; EC 6.3.2.4; MLP2DKFZp686E22157; MRP3MOAT-DEST90757; multidrug resistance associated protein; Multidrug resistance-associated protein 3; Multi-specific organic anion transporter D ABCC3 ABC31, EST90757, MLP2, MOAT-D, MRP3, cMOAT2 ATP binding cassette subfamily C member 3 ATP-binding cassette sub-family C member 3|ATP-binding cassette, sub-family C (CFTR/MRP), member 3|canalicular multispecific organic anion transporter 2|canicular multispecific organic anion transporter|multi-specific organic anion transporter D|multidrug resistance associated protein|multidrug resistance-associated protein 3

For more info on ABCC3, check out the ABCC3 Infographic

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In this infographic, you will see the following information for ABCC3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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