Anti-ABCG4 Antibody Picoband®

Product Name

Anti-ABCG4 Antibody Picoband®

View all ABCG4 Antibodies

SKU/Catalog Number

PA2067
BA3038 is an alternative SKU for this antibody, used in previous lots.

Size

100 μg/vial

Form

Lyophilized

Description

ABCG4 is an ATP-binding cassette (ABC) transporter (White subfamily) with expression and regulation consistent with macrophage cholesterol handling; Boster datasheet notes induction with LXR/RXR agonists and decreased expression after cholesterol removal, aligning with a role in lipid/cholesterol homeostasis (putative disease relevance in atherosclerosis/neuroinflammation). Assay context: rabbit polyclonal antibody validated for Western blotting (datasheet includes rat/mouse brain lysate WB image) with suggested 0.1–0.5 µg/mL; often contextualized with cholesterol-regulator PCSK9 or other endocytosis/trafficking markers like AP2B1 when studying LDLR/vesicular pathways (putative).

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ABCG4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2067)

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human ABCG4, different from the related mouse and rat sequences by one amino acid.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2067 is reactive to ABCG4 in Human, Mouse, Rat

Observed Molecular Weight

280 kDa

Calculated molecular weight

71.9 kDa

Background of ABCG4

ABCG4 (ATP-Binding Cassette, Subfamily G, Member 4), is a protein that in humans is encoded by the ABCG4 gene. The protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. This protein is a member of the White subfamily and is expressed predominantly in liver tissue. By genomic sequence analysis, Engel et al. (2001) mapped the ABCG4 gene to chromosome 11q23.3. Engel et al. (2001) demonstrated 5-fold induction of ABCG4 following treatment of normal monocyte-derived macrophages with the LXR and RXR agonists 9-cis retinoic acid and 22R hydroxycholesterol. Removal of cholesterol from macrophages by cyclodextrin decreased ABCG4 message levels.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA2067 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human

Positive Control

WB: Rat Brain Tissue, Mouse Brain Tissue,

Validation Images & Assay Conditions

See full target information ABCG4

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