Click image to see more details
Product Info Summary
| SKU: | A14869 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC |
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Product info
Product Name
Anti-Protein ABHD12B ABHD12B Antibody
SKU/Catalog Number
A14869
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Protein ABHD12B ABHD12B Antibody (Catalog# A14869). Tested in IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Protein ABHD12B ABHD12B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14869)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human ABHD12B. Immunogen sequence location: 281-330
Reactive Species
A14869 is reactive to ABHD12B in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
40.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14869 is guaranteed for ELISA, IF, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:100 - 1:300.
ELISA: 1:40000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using ABHD12B Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Protein ABHD12B ABHD12B Antibody (A14869)
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Customer Q&As
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5 Customer Q&As for Anti-Protein ABHD12B ABHD12B Antibody
Question
I was wanting to use to test anti-ABHD12B antibody A14869 on human skin for research purposes, then I may be interested in using anti-ABHD12B antibody A14869 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
The products we sell, including anti-ABHD12B antibody A14869, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-27
Question
Will A14869 anti-ABHD12B antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
F. Krishna
Verified customer
Asked: 2019-01-14
Answer
As indicated on the product datasheet, A14869 anti-ABHD12B antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-01-14
Question
We are currently using anti-ABHD12B antibody A14869 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
T. Parker
Verified customer
Asked: 2016-03-17
Answer
The anti-ABHD12B antibody (A14869) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-03-17
Question
Will anti-ABHD12B antibody A14869 work on canine IHC with skin?
K. Moore
Verified customer
Asked: 2014-10-27
Answer
Our lab technicians have not tested anti-ABHD12B antibody A14869 on canine. You can run a BLAST between canine and the immunogen sequence of anti-ABHD12B antibody A14869 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine skin in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-10-27
Question
Is a blocking peptide available for product anti-ABHD12B antibody (A14869)?
N. Li
Verified customer
Asked: 2014-04-14
Answer
We do provide the blocking peptide for product anti-ABHD12B antibody (A14869). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2014-04-14
