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- Table of Contents
Krt20 antibody (A02828-2) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Flow Cytometry analysis of CACO-2 cells using anti-Krt20 antibody (A02828-2).
Overlay histogram showing CACO-2 cells stained with A02828-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Krt20 Antibody (A02828-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing CACO-2 cells stained with A02828-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Krt20 Antibody (A02828-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Cytokeratin 20/Krt20 Antibody Picoband®
Krt20 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
Krt20 antibody was positively detected by WB in:
human RT4 whole cell, human Caco-2 whole cell, human Hela whole cell, human MCF-7 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Krt20 antibody was positively detected by IHC in:
human appendix tissue, rat intestine tissue, mouse intestine tissue, mouse bladder tissue, human stomach cancer tissue, human bladder cancer tissue, human pancreas cancer tissue,
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Krt20 antibody was positively detected by ICC/IF in:
A431 cell, NRK cell
Krt20 antibody was positively detected by FC in:
CACO-2 cell, Hela cell, RH35 cell
Krt20 antibody was positively detected by IF in:
human bladder cancer tissue, human stomach cancer tissue, human ovarian cancer tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.25μg/ml
Human, Mouse
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
2μg/ml
Human, Rat
Flow Cytometry (Fixed)|1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Tested
Recommend
Rat
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Product Information
Product Name
Anti-Cytokeratin 20/Krt20 Antibody Picoband®
View all KRT20 Antibodies
View all KRT20 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Cytokeratin 20/Krt20 recombinant protein (Position: R29-V431).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
48 kDa
Calculated Molecular Weight
49.0 kDa
Background of Krt20
Keratin 20, often abbreviated CK20, is a protein that in humans is encoded by the KRT20 gene. This gene encodes a member of the keratin protein family and is found within a cluster of cytokeratin genes on chromosome 11. Keratins are cytoskeletal proteins that are preferentially expressed in epithelial cells. The encoded protein may help maintain intermediate filament organization in intestinal epithelium. Phosphorylation of this protein may also influence mucin secretion in the small intestine.
Cite This Product
Anti-Cytokeratin 20/Krt20 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02828-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
91%
High Homology
Monkey
80%
Moderate Homology
Human
79%
Moderate Homology
Rabbit
75%
Moderate Homology
Cow
71%
Moderate Homology
Sheep
69%
Low homology
Dog
67%
Low homology
Zebrafish
52%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Krt20 antibody
Immunohistochemistry (IHC) protocol for Krt20 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for Krt20 antibody
Flow Cytometry (FC) protocol for Krt20 antibody
Immunofluorescence (IF) protocol for Krt20 antibody
Publications
Application:
Species:
Identification of relevant prognostic values of cytokeratin 20 and cytokeratin 7 expressions in lung cancer
Published: 2017-11-06
Journal: BIOSCIENCE REPORTS
Application: IHC
Impact Factor: 2.91
Species: Human
PMID: 28827446
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Product Promise
We promise Anti-Cytokeratin 20/Krt20 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A02828-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026