Product Info Summary
SKU: | A03668-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-Acetyl Coenzyme A Carboxylase/ACACB Antibody Picoband™
View all Acetyl Coenzyme A Carboxylase Beta Antibodies
SKU/Catalog Number
A03668-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Acetyl Coenzyme A Carboxylase/ACACB Antibody Picoband™ catalog # A03668-2. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Acetyl Coenzyme A Carboxylase/ACACB Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03668-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Acetyl Coenzyme A Carboxylase/ACACB, which shares 64.7% amino acid (aa) sequence identity with mouse Acetyl Coenzyme A Carboxylase/ACACB.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03668-2 is reactive to ACACB in Human, Mouse, Rat
Applications
A03668-2 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
277 kDa
Calculated molecular weight
276.541kDa
Background of Acetyl Coenzyme A Carboxylase Beta
Acetyl-CoA carboxylase 2 also known as ACC-beta or ACC2 is an enzyme that in humans is encoded by the ACACB gene. It is mapped to 12q24.11. Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. ACC-beta is thought to control fatty acid oxidation by means of the ability of malonyl-CoA to inhibit carnitine-palmitoyl-CoA transferase I, the rate-limiting step in fatty acid uptake and oxidation by mitochondria. ACC-beta may be involved in the regulation of fatty acid oxidation, rather than fatty acid biosynthesis. There is evidence for the presence of two ACC-beta isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Flow Cytometry, 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ACACB using anti-ACACB antibody (A03668-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat skeletal muscle tissue lysates
Lane 2: mouse skeletal muscle tissue lysates
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACACB antigen affinity purified polyclonal antibody (Catalog # A03668-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACACB at approximately 277KD. The expected band size for ACACB is at 277KD.
Click image to see more details
Figure 2. IHC analysis of ACACB using anti-ACACB antibody (A03668-2).
ACACB was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue se
Click image to see more details
Figure 3. Flow Cytometry analysis of HL-60 cells using anti-ACACB antibody (A03668-2).
Overlay histogram showing HL-60 cells stained with A03668-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACACB
Protein Target Info & Infographic
Gene/Protein Information For ACACB (Source: Uniprot.org, NCBI)
Gene Name
ACACB
Full Name
Acetyl-CoA carboxylase 2
Weight
276.541kDa
Alternative Names
ACC2ACC-beta; ACCBacetyl-CoA carboxylase 2; acetyl-CoA carboxylase beta; acetyl-Coenzyme A carboxylase beta; EC 6.4.1.2; HACC275 ACACB ACC-beta, ACC2, ACCB, HACC275 acetyl-CoA carboxylase beta acetyl-CoA carboxylase 2|acetyl-Coenzyme A carboxylase beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACACB, check out the ACACB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACACB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Acetyl Coenzyme A Carboxylase/ACACB Antibody Picoband™ (A03668-2)
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No publications found for A03668-2
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Customer Q&As
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1 Customer Q&As for Anti-Acetyl Coenzyme A Carboxylase/ACACB Antibody Picoband™
Question
We are currently using anti-Acetyl Coenzyme A Carboxylase/ACACB antibody A03668-2 for human tissue, and we are well pleased with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
J. Anderson
Verified customer
Asked: 2016-02-15
Answer
The anti-Acetyl Coenzyme A Carboxylase/ACACB antibody (A03668-2) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-02-15