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Product Info Summary
| SKU: | A09942 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Acetyl-Histone H2B (K126) HIST1H2BA Antibody
SKU/Catalog Number
A09942
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Acetyl-Histone H2B (K126) HIST1H2BA Antibody catalog # A09942. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Acetyl-Histone H2B (K126) HIST1H2BA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09942)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized Acetyl-peptide derived from human H2B around the Acetylation site of Lys126. AA range:78-127
Reactive Species
A09942 is reactive to HIST1H2BA in Human, Mouse, Rat
Calculated molecular weight
14.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09942 is guaranteed for ELISA, WB Boster Guarantee
Recommend Dilution
WB 1:500-1:2000
ELISA 1:20000
Validation Images & Assay Conditions
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Western Blot analysis of A549 cells using Acetyl-Histone H2B (K126) Polyclonal Antibody. Secondary antibody was diluted at 1:20000
Specific Publications For Anti-Acetyl-Histone H2B (K126) HIST1H2BA Antibody (A09942)
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Customer Q&As
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3 Customer Q&As for Anti-Acetyl-Histone H2B (K126) HIST1H2BA Antibody
Question
Will anti-Acetyl-Histone H2B (K126) antibody A09942 work for WB with testis?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
According to the expression profile of testis, HIST1H2BA is highly expressed in testis. So, it is likely that anti-Acetyl-Histone H2B (K126) antibody A09942 will work for WB with testis.
Boster Scientific Support
Answered: 2020-03-17
Question
Would A09942 anti-Acetyl-Histone H2B (K126) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
You can see on the product datasheet, A09942 anti-Acetyl-Histone H2B (K126) antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-05
Question
Would anti-Acetyl-Histone H2B (K126) antibody A09942 work on zebrafish WB with testis?
K. Williams
Verified customer
Asked: 2018-09-12
Answer
Our lab technicians have not validated anti-Acetyl-Histone H2B (K126) antibody A09942 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Acetyl-Histone H2B (K126) antibody A09942 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish testis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-09-12
