Product Info Summary
SKU: | M06819-11 |
---|---|
Size: | 100ug |
Reactive Species: | Human, Vertebrates |
Host: | Rabbit |
Application: | ChIP, ELISA, IHC, ICC, WB, Luminex |
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Product info
Product Name
Anti-Acetyl-Histone H3 (Lys9) H3F3A Rabbit Monoclonal Antibody, Clone#RM161
SKU/Catalog Number
M06819-11
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Acetyl-Histone H3 (Lys9) H3F3A Rabbit Monoclonal Antibody, Clone#RM161 (Catalog # M06819-11). Tested in WB, ICC, ChIP, IHC, ELISA, Luminex applications. This antibody reacts with Human, Vertebrates.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Acetyl-Histone H3 (Lys9) H3F3A Rabbit Monoclonal Antibody, Clone#RM161 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06819-11)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM161
Isotype
Rabbit IgG
Immunogen
An acetyl-peptide corresponding to the Acetyl-Histone H3 (Lys9)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross-reactivity with other acetylated Lysines in histone H3.
Reactive Species
M06819-11 is reactive to H3-3A in Human, Vertebrates
Applications
M06819-11 is guaranteed for ChIP, ELISA, IHC, ICC, WB, Luminex Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
15.328kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 0.25 ug/mL - 1 ug/mL
ICC: 0.5 ug/mL - 2 ug/mL
ChIP: 2 ug/mL-10 ug/mL
IHC: 0.1 ug/mL – 1 ug/mL
ELISA: 0.2 ug/mL - 1 ug/mL
Luminex: 0.05 ug/mL – 0.5 ug/mL.
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western Blotting result
Western Blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate, using RM161 at 0.25 ug/mL, showed a band of histone H3 acetylated at Lysine 9 in treated HeLa.
Click image to see more details
Figure 2. Specificity Test result
RM161 specifically reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.
Click image to see more details
Figure 3. IHC result
Immunohistochemistry staining of HepG2 cell using anti-Acetyl-Histone H3 (Lys9) antibody, RM161.
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Figure 4. ICC result
Immunocytochemistry of HeLa cells treated with sodium butyrate, using Acetyl-Histone H3 (Lys9) Rabbit mAb RM161 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
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Figure 5. ELISA result showing specificity
Sandwich ELISA against acetylated histone H3 at Lys 9 using HeLa whole cell lysate, treated or untreated with Sodium Butyrate. Using anti-H3CT (RM188, 1 ug/mL) as the capture antibody and biotinylated anti-H3K9ac (RM161, 1 ug/mL) as the detection antibody.
Click image to see more details
Figure 6. ChIP result
ChIP performed on HeLa cells using H3K9ac antibody (RM161, 5ug). Real-time PCR was performed using primers specific to the gene indicated.
Protein Target Info & Infographic
Gene/Protein Information For H3-3A (Source: Uniprot.org, NCBI)
Gene Name
H3-3A
Full Name
Histone H3.3
Weight
15.328kDa
Superfamily
histone H3 family
Alternative Names
Histone H3.3 H3-3A H3-3B, H3.3A, H3F3, H3F3A H3.3 histone A histone H3.3|H3 histone family member 3A|H3 histone, family 3A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on H3-3A, check out the H3-3A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for H3-3A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Acetyl-Histone H3 (Lys9) H3F3A Rabbit Monoclonal Antibody, Clone#RM161 (M06819-11)
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No publications found for M06819-11
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