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- Table of Contents
H3-3a antibody (M06819-30) performs well in WB and shows reactivity with Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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All lanes : Anti-H3f3b Antibody (C-Term) at 1:8000 dilution
Lane 1: NIH/3T3 whole cell lysate
Lane 2: mouse lung lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 15 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Lane 1: NIH/3T3 whole cell lysate
Lane 2: mouse lung lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 15 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Anti-H3f3b Antibody (C-Term)
H3-3a antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse | Rabbit | WB |
Recommend Dilution
Application
Dilution
Species
WB: 1:2000
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-H3f3b Antibody (C-Term)
View all H3-3A Antibodies
View all H3-3A Antibodies
Uniprot
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This H3f3b antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 103-136 amino acids from mouse H3f3b.
Calculated Molecular Weight
15.3 kDa
Background of H3-3a
Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cite This Product
Anti-H3f3b Antibody (C-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06819-30)
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Product Promise
We promise Anti-H3f3b Antibody (C-Term) performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
M06819-30
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026