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Product Info Summary
| SKU: | A08482 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-AChR alpha10 CHRNA10 Antibody
SKU/Catalog Number
A08482
Size
100μl
Form
Liquid
Description
Boster Bio Anti-AChR alpha10 CHRNA10 Antibody catalog # A08482. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AChR alpha10 CHRNA10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08482)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human CHRNA10. AA range:394-443
Reactive Species
A08482 is reactive to CHRNA10 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
49.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08482 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:20000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using AChRα10 Polyclonal Antibody
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Western Blot analysis of A549 cells using AChRα10 Polyclonal Antibody
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Western blot analysis of lysates from HeLa, 293, COLO, and A549 cells, using CHRNA10 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-AChR alpha10 CHRNA10 Antibody (A08482)
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Customer Q&As
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6 Customer Q&As for Anti-AChR alpha10 CHRNA10 Antibody
Question
I was wanting to use your anti-AChR alpha10 antibody for WB for human muscle of leg on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human muscle of leg identification?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
It shows on the product datasheet, A08482 anti-AChR alpha10 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human muscle of leg in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-24
Question
Is a blocking peptide available for product anti-AChR alpha10 antibody (A08482)?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
We do provide the blocking peptide for product anti-AChR alpha10 antibody (A08482). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-05-27
Question
We are currently using anti-AChR alpha10 antibody A08482 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
S. Bhatt
Verified customer
Asked: 2019-03-13
Answer
The anti-AChR alpha10 antibody (A08482) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-03-13
Question
Will anti-AChR alpha10 antibody A08482 work on dog WB with muscle of leg?
Verified Customer
Verified customer
Asked: 2018-12-18
Answer
Our lab technicians have not validated anti-AChR alpha10 antibody A08482 on dog. You can run a BLAST between dog and the immunogen sequence of anti-AChR alpha10 antibody A08482 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog muscle of leg in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-12-18
Question
Is there a BSA free version of anti-AChR alpha10 antibody A08482 available?
Verified Customer
Verified customer
Asked: 2018-09-28
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-AChR alpha10 antibody A08482 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-09-28
Question
My question regarding product A08482, anti-AChR alpha10 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Zhang
Verified customer
Asked: 2015-03-06
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A08482 anti-AChR alpha10 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-03-06
