Product Info Summary
SKU: | A02167-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-Acid phosphatase/ACP1 Antibody Picoband™
View all LMW-PTP/ACP1 Antibodies
SKU/Catalog Number
A02167-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Acid phosphatase/ACP1 Antibody Picoband™ catalog # A02167-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Acid phosphatase/ACP1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02167-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Acid phosphatase/ACP1 recombinant protein (Position: M1-H158).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02167-1 is reactive to ACP1 in Human, Mouse, Rat
Applications
A02167-1 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
17 kDa
Calculated molecular weight
18.042kDa
Background of LMW-PTP/ACP1
Low molecular weight phosphotyrosine protein phosphatase is an enzyme that in humans is encoded by the ACP1 gene. The product of this gene belongs to the phosphotyrosine protein phosphatase family of proteins. It functions as an acid phosphatase and a protein tyrosine phosphatase by hydrolyzing protein tyrosine phosphate to protein tyrosine and orthophosphate. This enzyme also hydrolyzes orthophosphoric monoesters to alcohol and orthophosphate. This gene is genetically polymorphic, and three common alleles segregating at the corresponding locus give rise to six phenotypes. Each allele appears to encode at least two electrophoretically different isozymes, Bf and Bs, which are produced in allele-specific ratios. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Acid phosphatase/ACP1 using anti-Acid phosphatase/ACP1 antibody (A02167-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human Hele whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: rat L6 whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: mouse 4T1 whole cell lysates,
Lane 7: mouse C2C12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Acid phosphatase/ACP1 antigen affinity purified polyclonal antibody (Catalog # A02167-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Acid phosphatase/ACP1 at approximately 17 kDa. The expected band size for Acid phosphatase/ACP1 is at 17 kDa.
Protein Target Info & Infographic
Gene/Protein Information For ACP1 (Source: Uniprot.org, NCBI)
Gene Name
ACP1
Full Name
Low molecular weight phosphotyrosine protein phosphatase
Weight
18.042kDa
Superfamily
low molecular weight phosphotyrosine protein phosphatase family
Alternative Names
acid phosphatase 1, soluble; acid phosphatase of erythrocyte; ACP1; Adipocyte acid phosphatase; cytoplasmic phosphotyrosyl protein phosphatase; EC 3.1.3.2; EC 3.1.3.48; HAAP; LMWPTP; LMW-PTP; LMW-PTPase; Low molecular weight cytosolic acid phosphatase; low molecular weight phosphotyrosine protein phosphatase; MGC111030; MGC3499; protein tyrosine phosphatase; Red cell acid phosphatase 1 ACP1 HAAP, LMW-PTP, LMWPTP acid phosphatase 1 low molecular weight phosphotyrosine protein phosphatase|LMW-PTPase|acid phosphatase 1, soluble|acid phosphatase of erythrocyte|adipocyte acid phosphatase|cytoplasmic phosphotyrosyl protein phosphatase|low molecular weight cytosolic acid phosphatase|protein tyrosine phosphatase|red cell acid phosphatase 1|testicular secretory protein Li 37
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACP1, check out the ACP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Acid phosphatase/ACP1 Antibody Picoband™ (A02167-1)
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