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Product Info Summary
| SKU: | A11589-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC |
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Product info
Product Name
Anti-ACOT12 Antibody
SKU/Catalog Number
A11589-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ACOT12 Antibody (Catalog# A11589-1). Tested in IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ACOT12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11589-1)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human ACOT12. Immunogen sequence location: 281-330
Reactive Species
A11589-1 is reactive to ACOT12 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
62.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11589-1 is guaranteed for ELISA, IF, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:40000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunofluorescence analysis of A549 cells, using ACOT12 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using ACOT12 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-ACOT12 Antibody (A11589-1)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-ACOT12 Antibody
Question
I was wanting to use your anti-ACOT12 antibody for IHC for rat liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat liver identification?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
It shows on the product datasheet, A11589-1 anti-ACOT12 antibody has been tested for IF, IHC on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-13
Question
Would anti-ACOT12 antibody A11589-1 work for IHC with liver?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
According to the expression profile of liver, ACOT12 is highly expressed in liver. So, it is likely that anti-ACOT12 antibody A11589-1 will work for IHC with liver.
Boster Scientific Support
Answered: 2019-10-09
Question
Is a blocking peptide available for product anti-ACOT12 antibody (A11589-1)?
N. Miller
Verified customer
Asked: 2019-05-16
Answer
We do provide the blocking peptide for product anti-ACOT12 antibody (A11589-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-05-16
Question
My lab would like to test anti-ACOT12 antibody A11589-1 on rat liver for research purposes, then I may be interested in using anti-ACOT12 antibody A11589-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-02-04
Answer
The products we sell, including anti-ACOT12 antibody A11589-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-02-04
Question
I see that the anti-ACOT12 antibody A11589-1 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-05-31
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-05-31
Question
We are currently using anti-ACOT12 antibody A11589-1 for rat tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
N. Miller
Verified customer
Asked: 2015-03-09
Answer
The anti-ACOT12 antibody (A11589-1) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-03-09
Question
Does anti-ACOT12 antibody A11589-1 work on zebrafish IF with right lobe of liver?
F. Gonzalez
Verified customer
Asked: 2015-01-22
Answer
Our lab technicians have not tested anti-ACOT12 antibody A11589-1 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-ACOT12 antibody A11589-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish right lobe of liver in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-01-22
