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ACSF2 antibody (A11449-1) performs well in ELISA, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of ACSF2 using anti-ACSF2 antibody (A11449-1).
ACSF2 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ACSF2 Antibody (A11449-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
ACSF2 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ACSF2 Antibody (A11449-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-ACSF2 Antibody Picoband®
ACSF2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IHC, WB |
Tested Applications
ACSF2 antibody was positively detected by WB in:
human RT4 whole cell, human HepG2 whole cell, human HEL whole cell, rat liver tissue, mouse liver tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ACSF2 antibody was positively detected by IHC in:
human kidney tissue, rat kidney tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human, Rat
ELISA
0.1-0.5 μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-ACSF2 Antibody Picoband®
View all ACSF2 Antibodies
View all ACSF2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human ACSF2 recombinant protein (Position: Q32-L615).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
60 kDa
Calculated Molecular Weight
68.1 kDa
Background of ACSF2
Enables medium-chain fatty acid-CoA ligase activity. Predicted to be involved in fatty acid metabolic process. Located in mitochondrion.
Cite This Product
Anti-ACSF2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11449-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
94%
High Homology
Cow
85%
Moderate Homology
Rabbit
85%
Moderate Homology
Sheep
85%
Moderate Homology
Dog
82%
Moderate Homology
Mouse
80%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ACSF2 antibody
Immunohistochemistry (IHC) protocol for ACSF2 antibody
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Product Promise
We promise Anti-ACSF2 Antibody Picoband® performs excellently in relevant applications for ELISA, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A11449-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 22, 2026