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Product Info Summary
| SKU: | M02809 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-ACSS2/Acetyl Coa Synthetase Rabbit Monoclonal Antibody
SKU/Catalog Number
M02809
BM4830 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ACSS2/Acetyl Coa Synthetase Rabbit Monoclonal Antibody catalog # M02809. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ACSS2/Acetyl Coa Synthetase Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02809)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
IEF-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ACSS2
Reactive Species
M02809 is reactive to ACSS2 in Human, Mouse, Rat
Observed Molecular Weight
79 kDa
Calculated molecular weight
78.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02809 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
Positive Control
WB: human K562 whole cell, human HepG2 whole cell, human HEL whole cell, rat liver tissue, rat kidney tissue, mouse liver tissue, mouse kidney tissue
Validation Images & Assay Conditions
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Western blot analysis of ACSS2 using anti-ACSS2 antibody (M02809).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human HEL whole cell lysates,
Lane 4: rat liver tissue lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: mouse liver tissue lysates,
Lane 7: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACSS2 antigen affinity purified monoclonal antibody (M02809) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACSS2 at approximately 79 kDa. The expected band size for ACSS2 is at 79 kDa.
Specific Publications For Anti-ACSS2/Acetyl Coa Synthetase Rabbit Monoclonal Antibody (M02809)
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Customer Q&As
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4 Customer Q&As for Anti-ACSS2/Acetyl Coa Synthetase Rabbit Monoclonal Antibody
Question
We are currently using anti-ACSS2/Acetyl Coa Synthetase Rabbit Monoclonal antibody M02809 for rat tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
The anti-ACSS2/Acetyl Coa Synthetase Rabbit Monoclonal antibody (M02809) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-09
Question
you antibody to test anti-ACSS2/Acetyl Coa Synthetase Rabbit Monoclonal antibody M02809 on rat cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-ACSS2/Acetyl Coa Synthetase Rabbit Monoclonal antibody M02809 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
The products we sell, including anti-ACSS2/Acetyl Coa Synthetase Rabbit Monoclonal antibody M02809, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-12
Question
What is the immunogen sequence/epitope information of M02809?
Verified customer
Asked: 2019-11-22
Answer
The immunogen sequence of the Anti-ACSS2/Acetyl Coa Synthetase Rabbit Monoclonal Antibody (M02809) is CYNVLDRNVHEKKLG.
Boster Scientific Support
Answered: 2019-11-25
Question
I was wanting to use your anti-ACSS2/Acetyl Coa Synthetase Rabbit Monoclonal antibody for WB for rat cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat cervix carcinoma erythroleukemia identification?
J. Evans
Verified customer
Asked: 2016-06-01
Answer
You can see on the product datasheet, M02809 anti-ACSS2/Acetyl Coa Synthetase Rabbit Monoclonal antibody has been tested for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-06-01
