Product Info Summary
SKU: | M00305 |
---|---|
Size: | 100ug/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Mouse |
Application: | ELISA, IHC |
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Product info
Product Name
Anti-ACTH (Adrenocorticotrophic Hormone) (N-Terminal) (Pituitary Marker) Monoclonal Antibody
SKU/Catalog Number
M00305
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-ACTH (Adrenocorticotrophic Hormone) (N-Terminal) (Pituitary Marker) Monoclonal Antibody (Catalog # M00305). Tested in ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-ACTH (Adrenocorticotrophic Hormone) (N-Terminal) (Pituitary Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00305)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: r57
Isotype
IgG1, kappa
Immunogen
N-terminal fragment of human ACTH conjugated to KLH
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00305 is reactive to POMC in Human, Mouse, Rat
Reconstitution
Reconstitute with 1ml water (15 minutes at room temperature).
Calculated molecular weight
29424 MW
Background of POMC
ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This monoclonal antibody is specific to Synacthen (aa1-24 of ACTH); does not react with CLIP (aa17-39 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs).It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00305 is guaranteed for ELISA, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA (For coating, order antibody without BSA)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
Click image to see more details
Formalin-fixed, paraffin-embedded human pituitary gland stained with Anti-ACTH Recombinant Mouse Monoclonal Antibody (r57).
Protein Target Info & Infographic
Gene/Protein Information For POMC (Source: Uniprot.org, NCBI)
Gene Name
POMC
Full Name
Pro-opiomelanocortin
Weight
29424 MW
Superfamily
POMC family
Alternative Names
Pro-opiomelanocortin;POMC;Corticotropin-lipotropin;NPP;Melanotropin gamma;Gamma-MSH;Potential peptide;Corticotropin;Adrenocorticotropic hormone;ACTH;Melanotropin alpha;Alpha-MSH;Corticotropin-like intermediary peptide;CLIP;Lipotropin beta;Beta-LPH;Lipotropin gamma;Gamma-LPH;Melanotropin beta;Beta-MSH;Beta-endorphin;Met-enkephalin;POMC; POMC ACTH, CLIP, LPH, MSH, NPP, OBAIRH, POC proopiomelanocortin pro-opiomelanocortin|adrenocorticotropic hormone|adrenocorticotropin|alpha-MSH|alpha-melanocyte-stimulating hormone|beta-LPH|beta-MSH|beta-endorphin|beta-melanocyte-stimulating hormone|corticotropin-like intermediary peptide|corticotropin-lipotropin|gamma-LPH|gamma-MSH|lipotropin beta|lipotropin gamma|melanotropin alpha|melanotropin beta|melanotropin gamma|met-enkephalin|opiomelanocortin prepropeptide|pro-ACTH-endorphin|proopiomelanocortin preproprotein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POMC, check out the POMC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POMC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ACTH (Adrenocorticotrophic Hormone) (N-Terminal) (Pituitary Marker) Monoclonal Antibody (M00305)
Hello CJ!
M00305 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Huo J, Liu J, Wang J, Zhang Y, Wang C, Yang Y, Sun W, Xu S. Neural Regen Res. 2012 Aug 5;7(22):1729-35. Doi: 10.3969/J.Issn.1673-5374.2012.22.008. Early Hyperbaric Oxygen Therapy Inhibits Aquaporin 4 And Adrenocorticotropic Hormone Expression In T...
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Customer Q&As
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3 Customer Q&As for Anti-ACTH (Adrenocorticotrophic Hormone) (N-Terminal) (Pituitary Marker) Monoclonal Antibody
Question
We have been able to see staining in rat pituitary gland. Are there any suggestions? Is anti-ACTH (Adrenocorticotrophic Hormone) (N-Terminal) (Pituitary Marker) Monoclonal antibody supposed to stain pituitary gland positively?
Verified Customer
Verified customer
Asked: 2018-10-23
Answer
From literature pituitary gland does express POMC. From Uniprot.org, POMC is expressed in pituitary gland, pituitary, among other tissues. Regarding which tissues have POMC expression, here are a few articles citing expression in various tissues:
Pituitary, Pubmed ID: 7828531, 15489334, 16807684
Boster Scientific Support
Answered: 2018-10-23
Question
My team were well pleased with the WB result of your anti-ACTH (Adrenocorticotrophic Hormone) (N-Terminal) (Pituitary Marker) Monoclonal antibody. However we have been able to see positive staining in pituitary gland secreted. using this antibody. Is that expected? Could you tell me where is POMC supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-05-14
Answer
From what I have seen in literature, pituitary gland does express POMC. Generally POMC expresses in secreted. Regarding which tissues have POMC expression, here are a few articles citing expression in various tissues:
Pituitary, Pubmed ID: 7828531, 15489334, 16807684
Boster Scientific Support
Answered: 2018-05-14
Question
We are currently using anti-ACTH (Adrenocorticotrophic Hormone) (N-Terminal) (Pituitary Marker) Monoclonal antibody M00305 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
C. Krishna
Verified customer
Asked: 2013-06-25
Answer
The anti-ACTH (Adrenocorticotrophic Hormone) (N-Terminal) (Pituitary Marker) Monoclonal antibody (M00305) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-06-25