Anti-ADAM17/Tace Rabbit Monoclonal Antibody

TACE/ADAM17 antibody

Boster Bio Anti-ADAM17/Tace Rabbit Monoclonal Antibody catalog # M00604. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M00604
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IP, IF, ICC, WB

Product Name

Anti-ADAM17/Tace Rabbit Monoclonal Antibody

View all TACE/ADAM17 Antibodies

SKU/Catalog Number

M00604

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-ADAM17/Tace Rabbit Monoclonal Antibody catalog # M00604. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ADAM17/Tace Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00604)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AOIO-1

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human ADAM17

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00604 is reactive to ADAM17 in Human, Mouse, Rat

Applications

M00604 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

93.021kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50
FC 1:100

Validation Images & Assay Conditions

Gene/Protein Information For ADAM17 (Source: Uniprot.org, NCBI)

Gene Name

ADAM17

Full Name

Disintegrin and metalloproteinase domain-containing protein 17

Weight

93.021kDa

Alternative Names

ADAM 17; ADAM metallopeptidase domain 17; ADAM metallopeptidase domain 18; ADAM17; ADAM18; CD156b antigen; CD156b; CSVP; disintegrin and metalloproteinase domain-containing protein 17; EC 3.4.24.86; MGC71942; Snake venom-like protease; TACE; TACEcSVP; TNF-alpha convertase; TNF-alpha converting enzyme; TNF-alpha-converting enzyme; tumor necrosis factor, alpha, converting enzyme ADAM17 ADAM18, CD156B, CSVP, NISBD, NISBD1, TACE ADAM metallopeptidase domain 17 disintegrin and metalloproteinase domain-containing protein 17|ADAM metallopeptidase domain 18|TNF-alpha convertase|TNF-alpha convertase enzyme|TNF-alpha converting enzyme|a disintegrin and metalloproteinase 17|cartilage snake venom-like protease|snake venom-like protease|tumor necrosis factor, alpha, converting enzyme

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ADAM17, check out the ADAM17 Infographic

ADAM17 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ADAM17: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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17 Customer Q&As for Anti-ADAM17/Tace Rabbit Monoclonal Antibody

Question

Is the immunogen sequence for M00604 raised against the N or C terminal?

Verified customer

Asked: 2022-05-16

Answer

The immunogen sequence for Anti-ADAM17/Tace Rabbit Monoclonal Antibody (M00604) was raised against the C terminal aa767-780.

Boster Scientific Support

Answered: 2022-05-17

Question

We have seen staining in human liver. Do you have any suggestions? Is anti-ADAM17/Tace Rabbit Monoclonal antibody supposed to stain liver positively?

Verified Customer

Verified customer

Asked: 2020-04-27

Answer

Based on literature liver does express ADAM17. Based on Uniprot.org, ADAM17 is expressed in oocyte, leukocyte monocyte, cartilage, cervix carcinoma, erythroleukemia, liver, among other tissues. Regarding which tissues have ADAM17 expression, here are a few articles citing expression in various tissues:
Cartilage, Pubmed ID: 9574564, 12058067
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Erythroleukemia, Pubmed ID: 23186163
Leukocyte, and Monocyte, Pubmed ID: 9034191
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2020-04-27

Question

We are currently using anti-ADAM17/Tace Rabbit Monoclonal antibody M00604 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-16

Answer

The anti-ADAM17/Tace Rabbit Monoclonal antibody (M00604) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-16

Question

Would anti-ADAM17/Tace Rabbit Monoclonal antibody M00604 work for Flow Cytometry with oocyte?

Verified Customer

Verified customer

Asked: 2020-03-20

Answer

According to the expression profile of oocyte, ADAM17 is highly expressed in oocyte. So, it is likely that anti-ADAM17/Tace Rabbit Monoclonal antibody M00604 will work for Flow Cytometry with oocyte.

Boster Scientific Support

Answered: 2020-03-20

Question

We ordered your anti-ADAM17/Tace Rabbit Monoclonal antibody for IP on erythroleukemia a few months ago. I am using rat, and We are going to use the antibody for ICC next. I am interested in examining erythroleukemia as well as liver in our next experiment. Could you please give me some suggestion on which antibody would work the best for ICC?

Verified Customer

Verified customer

Asked: 2020-01-24

Answer

I have checked the website and datasheets of our anti-ADAM17/Tace Rabbit Monoclonal antibody and I see that M00604 has been tested on rat in both IP and ICC. Thus M00604 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-01-24

Question

Does M00604 anti-ADAM17/Tace Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-10-11

Answer

You can see on the product datasheet, M00604 anti-ADAM17/Tace Rabbit Monoclonal antibody as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-10-11

Question

I see that the anti-ADAM17/Tace Rabbit Monoclonal antibody M00604 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-08-21

Answer

You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-08-21

Question

See attached the WB image, lot number and protocol we used for oocyte using anti-ADAM17/Tace Rabbit Monoclonal antibody M00604. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-08-15

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-15

Question

I was wanting to use using your anti-ADAM17/Tace Rabbit Monoclonal antibody for positive regulation of g1/s transition of mitotic cell cycle studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-08-12

Answer

Thanks for your inquiry. This M00604 anti-ADAM17/Tace Rabbit Monoclonal antibody is tested on hela cells. It is guaranteed to work for Flow Cytometry, IP, IF, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-08-12

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for oocyte using anti-ADAM17/Tace Rabbit Monoclonal antibody M00604. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-05-13

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-05-13

Question

My boss were well pleased with the WB result of your anti-ADAM17/Tace Rabbit Monoclonal antibody. However we have seen positive staining in leukocyte monocyte membrane using this antibody. Is that expected? Could you tell me where is ADAM17 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-11-16

Answer

From literature, leukocyte monocyte does express ADAM17. Generally ADAM17 expresses in membrane. Regarding which tissues have ADAM17 expression, here are a few articles citing expression in various tissues:
Cartilage, Pubmed ID: 9574564, 12058067
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Erythroleukemia, Pubmed ID: 23186163
Leukocyte, and Monocyte, Pubmed ID: 9034191
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2018-11-16

Question

Is this M00604 anti-ADAM17/Tace Rabbit Monoclonal antibody reactive to the isotypes of ADAM17?

Verified Customer

Verified customer

Asked: 2018-09-27

Answer

The immunogen of M00604 anti-ADAM17/Tace Rabbit Monoclonal antibody is A synthesized peptide derived from human ADAM17. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-09-27

Question

I would like to test anti-ADAM17/Tace Rabbit Monoclonal antibody M00604 on human oocyte for research purposes, then I may be interested in using anti-ADAM17/Tace Rabbit Monoclonal antibody M00604 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-09-14

Answer

The products we sell, including anti-ADAM17/Tace Rabbit Monoclonal antibody M00604, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-09-14

Question

Is a blocking peptide available for product anti-ADAM17/Tace Rabbit Monoclonal antibody (M00604)?

Verified Customer

Verified customer

Asked: 2018-06-19

Answer

We do provide the blocking peptide for product anti-ADAM17/Tace Rabbit Monoclonal antibody (M00604). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-06-19

Question

Is there a BSA free version of anti-ADAM17/Tace Rabbit Monoclonal antibody M00604 available?

L. Roberts

Verified customer

Asked: 2017-08-04

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ADAM17/Tace Rabbit Monoclonal antibody M00604 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-08-04

Question

Can you help my question with product M00604, anti-ADAM17/Tace Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

T. Edwards

Verified customer

Asked: 2017-03-23

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00604 anti-ADAM17/Tace Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-03-23

Question

I was wanting to use your anti-ADAM17/Tace Rabbit Monoclonal antibody for Flow Cytometry for human oocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human oocyte identification?

J. Collins

Verified customer

Asked: 2013-01-21

Answer

You can see on the product datasheet, M00604 anti-ADAM17/Tace Rabbit Monoclonal antibody has been validated for Flow Cytometry, IP, IF, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human oocyte in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-01-21

Size

Total: $370

SKU:M00604

In stock, 1 left.

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M00604
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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