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Product Info Summary
| SKU: | A07531 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-ADAM29 Antibody
SKU/Catalog Number
A07531
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ADAM29 Antibody (Catalog # A07531). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
ADAM29 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-ADAM29 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07531)
Host
Rabbit
Contents
ADAM29 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ADAM29 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human ADAM29. The immunogen is located within amino acids 710 - 760 of ADAM29.
Cross-reactivity
ADAM29 antibody is human specific. ADAM29 antibody is predicted to not cross-react with other ADAM family proteins.
Reactive Species
A07531 is reactive to ADAM29 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
92.8 kDa
Background of ADAM29
The ADAM (a disintegrin and metalloprotease) protein family plays a role in cell-cell and cell-matrix interface related processes, including fertilization, muscle fusion, secretion of TNFalpha (tumor necrosis factor alpha), and modulation of the neurogenic function of Notch and Delta (1-3). ADAM29 contains one disintegrin domain, one EGF-like domain and one peptidase M12B domain (2). It is highly expressed in testes (3) and may be involved in spermatogenesis and fertilization (4,5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07531 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ADAM29 antibody can be used for detection of ADAM29 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ADAM29 in human bladder tissue lysate with ADAM29 antibody at (A) 1 and (B) 2 μg/ml.
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Immunohistochemistry of ADAM29 in human testis tissue with ADAM29 antibody at 5 μg/mL.
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Immunofluorescence of ADAM29 in human testis tissue with ADAM29 antibody at 20 μg/mL.
Specific Publications For Anti-ADAM29 Antibody (A07531)
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