Rabbit IgG polyclonal antibody for A disintegrin and metalloproteinase with thrombospondin motifs 2(ADAMTS2) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat.
Anti-ADAMTS2 Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
CST4 is a member of the cystatin superfamily which contains proteins that hold multiple cystatin-like sequences. Several family members are active cysteine protease inhibitors, whereas others have lost or possibly never attained this inhibitory activity. CST4 strongly inhibits papain (non-competitively) and ficin, partially inhibits stem bromelain and bovine cathepsin C, however does not inhibit porcine cathepsin B or clostripain.
|Product Name||Anti-ADAMTS2 Antibody
See all ADAMTS2 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for ADAMTS 2/ADAMTS2 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. ADAMTS 2/ADAMTS2 information: Molecular Weight: 134755 MW; Subcellular Localization: Secreted, extracellular space, extracellular matrix ; Tissue Specificity: Expressed at high level in skin, bone, tendon and aorta and at low levels in thymus and brain.|
|Cite This Product||Anti-ADAMTS2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1461)|
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|Anti-ADAMTS2 Antibody may replace the following items: sc 100479|sc 134655|sc 134656|sc 162486|sc 162487|sc 393562.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human ADAMTS2(1189-1204aa KTRNQRIQELIDEMRK), different from the related rat and mouse sequences by three amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For ADAMTS2 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||9509|
|Species Of This Entry||Human|
|Protein Name||A disintegrin and metalloproteinase with thrombospondin motifs 2|
|Alternative Names||ADAMTS2|A disintegrin and metalloproteinase with thrombospondin motifs 2; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 2; ADAM metallopeptidase with thrombospondin type 1 motif, 2; ADAM-TS 2; ADAMTS-2; ADAM-TS2EC 220.127.116.11; ADAMTS-3; EC 3.4.24; NPIDKFZp686F12218; PC I-NP; PCI-NP; PCINPhPCPNI; PCPNI; PNPI; Procollagen I N-proteinase; Procollagen I/II amino propeptide-processing enzyme; Procollagen N-endopeptidase|
|Post Tranlational Modifications||Cleavage|
|Subcellular Localization||Secreted, extracellular space, extracellular matrix.|
|Gene Location||5q35.3, on Chromasome 5, gene sequence: NC_000005.10 (179110853..179345461, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For ADAMTS2 (Source: Uniprot.org, NCBI)
|Protein Function||Cleaves the propeptides of type I and II collagen prior to fibril assembly. Does not act on type III collagen. May also play a role in development that is independent of its role in collagen biosynthesis.|
|Tissue Specificity||Expressed at high level in skin, bone, tendon and aorta and at low levels in thymus and brain.|
|Research Areas||Angiogenesis; Cancer; Extracellular Matrix|
|Related Diseases||Ehlers-danlos Syndrome; Tumor Angiogenesis; Malignant Neoplasms; Ehlers-danlos Syndrome, Dermatosparaxis Type; Neoplasms; Degenerative Polyarthritis; Collagen Diseases; Carcinoma; Dysplasia; Thrombocytopenic Purpura; Hereditary Diseases; Malignant Paraganglionic Neoplasm; Connective Tissue Diseases; Purpura, Thrombotic Thrombocytopenic; Fracture; Tissue Adhesions; Premature Birth; Purpura; Neoplasm Metastasis; Adenoma|
|Related Pathways||Angiogenesis; Coagulation; Cell Migration; Immune Response; Proteolysis; Cell-matrix Adhesion; Muscle Contraction; Embryo Development; Reverse Transcription; Dentinogenesis; Cell Cycle; Pathogenesis; Tissue Homeostasis; Secretion|
|Background||ADAMTS2, A disintegrin and metalloproteinase with thrombospondin motifs 2, also known as procollagen I N-proteinase(PC I-NP), is an enzyme that in humans is encoded by the ADAMTS2 gene. The ADAMTS2 gene contains 22 exons. The human ADAMTS2 gene is mapped to chromosome 5q23-q24 by analysis of somatic cell hybrids. ADAMTS2 is responsible for processing several types of procollagen proteins. Procollagens are the precursors of collagens, the proteins that add strength and support to many body tissues. Specifically, this enzyme clips a short chain of amino acids off one end of the procollagen. This clipping step is necessary for collagen molecules to function normally and assemble into fibrils outside cells.|
|Scientific References||PMID: 10417273 by Colige A., et al. Human Ehlers-Danlos syndrome type VII C and bovine dermatosparaxis are caused by mutations in the procollagen I N-proteinase gene.|
PMID: 31152061 by Rosell-Garcia T., et al. Differential cleavage of lysyl oxidase by the metalloproteinases BMP1 and ADAMTS2/14 regulates collagen binding through a tyrosine sulfate domain.
Our Boster Quality Guarantee for Anti-ADAMTS2 Antibody covers its use in the following applications.Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Rat, Human, Mouse
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Heart Tissue Lysate
IHC(P): Rat Cardiac Muscle Tissue
IHC(F): Rat Cardiac Muscle Tissue
IHC(F): Rat Trachea Tissue
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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