Anti-ADAR1 Antibody Picoband™

Product Name

Anti-ADAR1 Antibody Picoband™

View all ADAR Antibodies

SKU/Catalog Number

PB9976

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ADAR1 Antibody Picoband™ catalog # PB9976. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ADAR1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9976)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human ADAR1 recombinant protein (Position: S128-Q346). Human ADAR1 shares 90.2% and 50.7% amino acid (aa) sequence identity with mouse and rat ADAR1, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9976 is reactive to ADAR in Human

Applications

PB9976 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

110 kDa, 150 kDa

Calculated molecular weight

136.066kDa

Background of ADAR

Double-stranded RNA-specific adenosine deaminase, also known as ADAR1, is an enzyme that in humans is encoded by the ADAR gene. It is mapped to 1q21.3. This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of ADAR1 using anti-ADAR1 antibody (PB9976).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates,
Lane 2: A549 whole cell lysates
Lane 3: MCF-7 whole cell lysates
Lane 4: HEPG2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAR1 antigen affinity purified polyclonal antibody (Catalog # PB9976) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAR1 at approximately 110KD, 150KD. The expected band size for ADAR1 is at 136KD.

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Figure 2. IHC analysis of ADAR1 using anti-ADAR1 antibody (PB9976).
ADAR1 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ADAR1 Antibody (PB9976) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Gene/Protein Information For ADAR (Source: Uniprot.org, NCBI)

Alternative Names

ADAR1DSH; adenosine deaminase, RNA-specific; DRADAP136; DSRADadenosine deaminase acting on RNA 1-A; EC 3.5.4; EC 3.5.4.-; G1P1double-stranded RNA-specific adenosine deaminase; IFI-4; IFI4dsRNA adenosine deaminase; interferon-induced protein 4; Interferon-inducible protein 4; K88DSRBP; p136,136 kDa double-stranded RNA-binding protein ADAR ADAR1, AGS6, DRADA, DSH, DSRAD, G1P1, IFI-4, IFI4, K88DSRBP, P136 adenosine deaminase RNA specific double-stranded RNA-specific adenosine deaminase|136 kDa double-stranded RNA-binding protein|adenosine deaminase acting on RNA 1-A|dsRNA adenosine deaminase|dsRNA adeonosine deaminase|interferon-induced protein 4|interferon-inducible protein 4

For more info on ADAR, check out the ADAR Infographic

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In this infographic, you will see the following information for ADAR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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