Anti-ADD1 (Ab-726) Antibody

Product Name

Anti-ADD1 (Ab-726) Antibody

View all alpha Adducin Antibodies

SKU/Catalog Number

A01575

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-ADD1 (Ab-726) Antibody (Catalog # A01575). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ADD1 (Ab-726) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01575)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized non-phosphopeptide derived from human ADD1 around the phosphorylation site of serine 726 (T-P-S(p)-F-L).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01575 is reactive to ADD1 in Human, Mouse, Rat

Applications

A01575 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

80.955kDa

Background of alpha Adducin

Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.

Yun Sok Lee, Mol. Cell. Biol., Jan 2007; 27: 438 - 452.
Kang Ho Kim, J. Biol. Chem., Dec 2004; 279: 51999 - 52006.
Marc Foretz, Mol. Cell. Biol., May 1999; 19: 3760 - 3768.
Jae Bum Kim, J. Clin. Invest., Jan 1998; 101: 1.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500

Validation Images & Assay Conditions

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Immunohistochemical analysis of paraffin-embedded human brain tissue using ADD1 (Ab-726) antibody A01575.

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Western blot analysis of extracts from Hela cells treated with Forskolin (40nM, 30min), using ADD1 (Ab-726) antibody A01575.

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Immunofluorescence analysis of HeLa cells, using ADD1 (Ab-726) antibody A01575

Gene/Protein Information For ADD1 (Source: Uniprot.org, NCBI)

Alternative Names

ADDA; adducin 1 (alpha); alpha-adducin; erythrocyte adducin alpha subunit; Erythrocyte adducin subunit alpha; MGC3339; MGC44427 ADD1 ADDA adducin 1 alpha-adducin|adducin 1 (alpha)|erythrocyte adducin alpha subunit

For more info on ADD1, check out the ADD1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ADD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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