Alpha-adducin Antibodies

Alpha-adducin (ADD1)

Membrane-cytoskeleton-associated protein that promotes the assembly of this spectrin-actin network. Binds to calmodulin.

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Gene Information

Human
Gene Name:	ADD1
Uniprot:	P35611
Entrez:	118

Family

Belongs to:
aldolase class II family

Alternative Names

ADDA; adducin 1 (alpha); alpha-adducin; erythrocyte adducin alpha subunit; Erythrocyte adducin subunit alpha; MGC3339; MGC44427

Molecular Weight

Mass (kDA):
80.955 kDA

Genomic Context

Human
Location:	4p16.3
Sequence:	4; NC_000004.12 (2843844..2930076)

Tissue Specificity

Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.

Subcellular Localizations

Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side.

Diseases

• Hypertensive Disease
• Essential Hypertension
• Cardiovascular Diseases
• Obesity
• Cerebrovascular Accident
• Diabetes Mellitus
• Infarction
• Insulin Resistance
• Myocardial Infarction
• Insulin Sensitivity
• Diabetes Mellitus, Non-insulin-dependent
• Cholangiocarcinoma
• Atherosclerosis

• Coronary Artery Disease
• Hemorrhage
• Huntington Disease
• 5,10-methylenetetrahydrofolate Reductase Deficiency
• Heart Diseases
• Ischemic Stroke
• Kidney Diseases

Interacting Proteins

• HMG20A

Pathways

• Transport
• Energy Homeostasis
• Pathogenesis
• Cholesterol Homeostasis
• Excretion
• Cell Differentiation
• Fatty Acid Oxidation
• Regulation Of Gene Expression
• Tissue Development
• Reverse Transcription
• Glucose Transport
• Cell Proliferation
• Adipose Tissue Development

• Angiogenesis
• Germination
• Fat Cell Differentiation
• Secretion
• Stem Cell Differentiation
• Osteoblast Differentiation
• Shoot Development

PTMs

• Phosphorylation
• Oxidation

• Cleavage
• Acetylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/ADD1

The Best Uses Of The ADD1 Marker

You've found the right place, whether you're looking to find a high-affinity antibody or a gene informationgraphic. This article covers Steven Boster and the ADD1 marker. Read on to learn more about the products available to you and the different uses of the ADD1 marker. ADD1 is a gene that is involved in the development of Alzheimer's disease and other neurodegenerative diseases.

Steven Boster

Steve Boster's greatest achievement is using the ADD1 marker. He was the highest scorer in the MMORPG (Massively Multiplayer Online Roleplaying Game). He had also reached the pinnacle of the game's level cap, which is a feat that is difficult to accomplish. He didn't stop there. He went on to use the ADD1 marker in the best possible way.

High-affinity primary antibodies

A monoclonal antibody binds to an epitope inside a fixed tissue and is called a high affinity primary antibody. The ratio of koff/kon determines the affinity constant. The higher the KD, the greater the antibody's affinity. This study was carried out at the University of California at Davis. Scientists at Abcam analysed the data.

One of the challenges of using an antibody is avoiding cross-reactivity between the two antibodies. Double staining can occur due to their different subtypes. Researchers can use secondary antibodies to recognize each subtype of each antibody. A primary antibody can also be labeled directly using an activated fluorescent color if the protein of concern is abundant.

You can confirm the specificity by using a worm specific method for affinity destruction. You can use transgenic overexpression lines as a positive control for these experiments. Nonspecific binding can also be tested using a worm-specific method. A high-affinity primary antibody will be less likely to react with the ADD1 protein than a nonspecific antibody.

An affinity depletion protocol is another way to determine affinity. This technique can be used with either partially purified antigen proteins or non-pure fusion proteins. The proteins are then combined and Western-blotted using a high binding nitrocellulose membrane. This allows for the cutting of the desired area. These results are available on the product pages.

One high-affinity antibody against the ADD1 genes recognizes the same epitope that the clone 12CA5. Its low working concentrations make it ideal for ELISA applications, western blotting, and other immunocytochemistry experiments. An antibody specific to the ADD1 marker can be used to screen the worm. An antibody with high affinity for the ADD1 mark may not be suitable to in situ applications. However, it will work in Western blotting for certain cases.

Primary antibodies can be prepared very similarly to secondary antibodies. A Y-shaped molecule consists of two arms with a flexible hinge between the stalk and arms. The stalk is the structural and functional component of the antibody and determines its isotype and functional properties. Each arm contains a lighter and a heavier chain. The heavy chain has a variable domain and a constant domain, and each of the two has an epitope-specific region.

ADD1 marker

Boster Bio has an ELISA kit that can be used to analyze a cell for ADD1 levels. The ELISA Kit from Boster Bio features an easy to use, high throughput approach that allows you to monitor ADD1/2 protein expression profiles in cells. It is also capable of measuring the relative levels ADD1/2 in cultured cells. This makes ELISA a great tool for screening applications and testing various treatments and activators.

Gene infographics

Check out the gene infographics based in this particular gene to learn more information about the ADD1 mark. These images provide basic data on all genes. All genes are covered in the infographics, both for humans and mice. There is also a search option that will allow you to find the gene you are interested. Below are three gene infographics that use the ADD1 marker.