Product Info Summary
SKU: | A05046 |
---|---|
Size: | 100μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Adducin gamma ADD3 Antibody
View all gamma Adducin Antibodies
SKU/Catalog Number
A05046
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Adducin gamma ADD3 Antibody catalog # A05046. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Adducin gamma ADD3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05046)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
5G12G2
Isotype
IgG
Immunogen
Recombinant protein within human USP28 aa 550-700.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05046 is reactive to ADD3 in Human, Mouse, Rat
Applications
A05046 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
79.155kDa
Background of gamma Adducin
May play an important role in cell fate determination during lung development and in spermatogenesis.
Li C.-S., Chae S.-C., Lee J.-H., Zhang Q., Chung H.-T.J. Hum. Genet. 51:292-297(2006)
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of HepG2 cells using Adducin gamma Polyclonal antibody.
Click image to see more details
Western Blot (WB) analysis of specific cells using Adducin gamma Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For ADD3 (Source: Uniprot.org, NCBI)
Gene Name
ADD3
Full Name
Gamma-adducin
Weight
79.155kDa
Superfamily
aldolase class II family
Alternative Names
ADDLAdducin-like protein 70; adducin 3 (gamma); gamma-adducin ADD3 ADDL, CPSQ3 adducin 3 gamma-adducin|adducin 3 (gamma)|adducin-like protein 70
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ADD3, check out the ADD3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ADD3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Adducin gamma ADD3 Antibody (A05046)
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No publications found for A05046
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Customer Reviews
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Customer Q&As
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12 Customer Q&As for Anti-Adducin gamma ADD3 Antibody
Question
I was wanting to use your anti-Adducin gamma antibody for WB for rat cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat cervix carcinoma erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
As indicated on the product datasheet, A05046 anti-Adducin gamma antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-22
Question
Is a blocking peptide available for product anti-Adducin gamma antibody (A05046)?
P. Zhao
Verified customer
Asked: 2019-12-30
Answer
We do provide the blocking peptide for product anti-Adducin gamma antibody (A05046). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-30
Question
Do you have a BSA free version of anti-Adducin gamma antibody A05046 available?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Adducin gamma antibody A05046 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-24
Question
Will A05046 anti-Adducin gamma antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
It shows on the product datasheet, A05046 anti-Adducin gamma antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-19
Question
I would like to test anti-Adducin gamma antibody A05046 on rat cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-Adducin gamma antibody A05046 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
The products we sell, including anti-Adducin gamma antibody A05046, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-08
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Adducin gamma antibody A05046. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-04-25
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-04-25
Question
Here is the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Adducin gamma antibody A05046. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-07-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-24
Question
My question regarding product A05046, anti-Adducin gamma antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-03-19
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05046 anti-Adducin gamma antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-03-19
Question
I see that the anti-Adducin gamma antibody A05046 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-01-12
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-01-12
Question
We are currently using anti-Adducin gamma antibody A05046 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
S. Baker
Verified customer
Asked: 2015-01-19
Answer
The anti-Adducin gamma antibody (A05046) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-01-19
Question
Does anti-Adducin gamma antibody A05046 work on canine WB with cervix carcinoma erythroleukemia?
Z. Anderson
Verified customer
Asked: 2014-02-10
Answer
Our lab technicians have not validated anti-Adducin gamma antibody A05046 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Adducin gamma antibody A05046 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-02-10
Question
Will anti-Adducin gamma antibody A05046 work for WB with cervix carcinoma erythroleukemia?
P. Baker
Verified customer
Asked: 2013-10-02
Answer
According to the expression profile of cervix carcinoma erythroleukemia, ADD3 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-Adducin gamma antibody A05046 will work for WB with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2013-10-02