Product Info Summary
SKU: | PB10030 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-ADH4 Antibody Picoband™
View all alcohol dehydrogenase 4 Antibodies
SKU/Catalog Number
PB10030
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ADH4 Antibody Picoband™ catalog # PB10030. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ADH4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10030)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human ADH4 recombinant protein (Position: K218-F380). Human ADH4 shares 68.7% and 71.8% amino acid (aa) sequence identity with mouse and rat ADH4, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB10030 is reactive to ADH4 in Human, Mouse, Rat
Applications
PB10030 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
40 kDa
Calculated molecular weight
40.222kDa
Background of alcohol dehydrogenase 4
Alcohol dehydrogenase 4 is an enzyme that in humans is encoded by the ADH4 gene. This gene encodes class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole. This gene is localized to chromosome 4 in the cluster of alcohol dehydrogenase genes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ADH4 using anti-ADH4 antibody (PB10030).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse liver tissue lysates.
Lane 3: HEPG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADH4 antigen affinity purified polyclonal antibody (Catalog # PB10030) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADH4 at approximately 40 kDa. The expected band size for ADH4 is at 40 kDa.
Protein Target Info & Infographic
Gene/Protein Information For ADH4 (Source: Uniprot.org, NCBI)
Gene Name
ADH4
Full Name
All-trans-retinol dehydrogenase [NAD(+)] ADH4
Weight
40.222kDa
Superfamily
zinc-containing alcohol dehydrogenase family
Alternative Names
ADH-2; alcohol dehydrogenase 4 (class II), pi polypeptide; alcohol dehydrogenase 4; Alcohol dehydrogenase class II pi chain; aldehyde reductase; EC 1.1.1; EC 1.1.1.1 ADH4 ADH-2, HEL-S-4 alcohol dehydrogenase 4 (class II), pi polypeptide all-trans-retinol dehydrogenase [NAD(+)] ADH4|alcohol dehydrogenase class II pi chain|aldehyde reductase|epididymis secretory protein Li 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ADH4, check out the ADH4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ADH4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ADH4 Antibody Picoband™ (PB10030)
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No publications found for PB10030
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-ADH4 Antibody Picoband™
Question
We are currently using anti-ADH4 antibody PB10030 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
The anti-ADH4 antibody (PB10030) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-25