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Product Info Summary
| SKU: | A04571 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, ICC |
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Product info
Product Name
Anti-Alpha-1D adrenergic receptor ADRA1D Antibody
View all alpha-1D Adrenergic R/ADRA1D Antibodies
SKU/Catalog Number
A04571
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Alpha-1D adrenergic receptor ADRA1D Antibody (Catalog# A04571). Tested in IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Rat, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Alpha-1D adrenergic receptor ADRA1D Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04571)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
7A7
Isotype
Rabbit IgG
Immunogen
A peptide derived from human ADRA1D. Immunogen sequence location: 451-500
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A04571 is reactive to ADRA1D in Human, Mouse, Rat
Applications
A04571 is guaranteed for ELISA, IF, IHC-P, ICC Boster Guarantee
Observed Molecular Weight
72 kDa
Calculated molecular weight
60.463kDa
Background of alpha-1D Adrenergic R/ADRA1D
Integrin alpha 1 (ITGA1) chain associates with the beta 1 (ITGB1) chain to form a heterodimer that functions as a dual laminin/collagen receptor in neural cells and hematopoietic cells. ITGA1 has a 206-amino acid I domain in its N-terminal half, followed by 3 divalent cation-binding sites and a C-terminal transmembrane domain with a short cytoplasmic tail. It also has 28 potential N-glycosylation sites. Human ITGA1 was expressed in a mouse fibroblast cell line as a 180-kD protein. ITGA1 is involved in the early remodeling of osteoarthritic cartilage and plays an essential role in the regulation of mesenchymal stem cell proliferation and cartilage production. It also plays an essential role in the regulation of MSC proliferation and cartilage production.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:20000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunofluorescence analysis of HeLa cells, using ADRA1D Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using ADRA1D Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For ADRA1D (Source: Uniprot.org, NCBI)
Gene Name
ADRA1D
Full Name
Alpha-1D adrenergic receptor
Weight
60.463kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
a-1D AdrenergicR; ADRA1; ADRA1ADAR; ADRA1D; ADRA1R; adrenergic, alpha -1D-, receptor; adrenergic, alpha-1A-, receptor; adrenergic, alpha-1D-, receptor; ALPHA1; Alpha-1A adrenergic receptor; alpha-1D Adrenergic R; alpha-1D adrenergic receptor; alpha1D AdrenergicR; Alpha-1D adrenoceptor; Alpha-1D adrenoreceptor; alpha-1D-adrenergic receptor; Alpha-adrenergic receptor 1a; DAR; dJ779E11.2 ADRA1D ADRA1, ADRA1A, ADRA1R, ALPHA1, DAR, dJ779E11.2 adrenoceptor alpha 1D alpha-1D adrenergic receptor|adrenergic, alpha -1D-, receptor|adrenergic, alpha-1A-, receptor|alpha-1A adrenergic receptor|alpha-1D adrenoceptor|alpha-1D adrenoreceptor|alpha-adrenergic receptor 1a
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ADRA1D, check out the ADRA1D Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ADRA1D: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Alpha-1D adrenergic receptor ADRA1D Antibody (A04571)
Hello CJ!
No publications found for A04571
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Recommended Resources
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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13 Customer Q&As for Anti-Alpha-1D adrenergic receptor ADRA1D Antibody
Question
Will anti-ADRA1D antibody A04571 work for IHC with placenta prostate?
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
According to the expression profile of placenta prostate, ADRA1D is highly expressed in placenta prostate. So, it is likely that anti-ADRA1D antibody A04571 will work for IHC with placenta prostate.
Boster Scientific Support
Answered: 2019-12-24
Question
Our lab want to know about to test anti-ADRA1D antibody A04571 on human placenta prostate for research purposes, then I may be interested in using anti-ADRA1D antibody A04571 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
The products we sell, including anti-ADRA1D antibody A04571, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-03
Question
I see that the anti-ADRA1D antibody A04571 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-05
Question
Does A04571 anti-ADRA1D antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
You can see on the product datasheet, A04571 anti-ADRA1D antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-25
Question
Is a blocking peptide available for product anti-ADRA1D antibody (A04571)?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
We do provide the blocking peptide for product anti-ADRA1D antibody (A04571). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-25
Question
Do you have a BSA free version of anti-ADRA1D antibody A04571 available?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ADRA1D antibody A04571 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-24
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta prostate using anti-ADRA1D antibody A04571. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-29
Question
Is this A04571 anti-ADRA1D antibody reactive to the isotypes of ADRA1D?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
The immunogen of A04571 anti-ADRA1D antibody is A peptide derived from human ADRA1D. Immunogen sequence location: 451-500. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-16
Question
I have attached the WB image, lot number and protocol we used for placenta prostate using anti-ADRA1D antibody A04571. Please let me know if you require anything else.
C. Mitchell
Verified customer
Asked: 2019-03-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-03-18
Question
I was wanting to use your anti-ADRA1D antibody for IHC for human placenta prostate on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human placenta prostate identification?
E. Collins
Verified customer
Asked: 2017-09-21
Answer
It shows on the product datasheet, A04571 anti-ADRA1D antibody has been tested for IF, IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human placenta prostate in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-09-21
Question
My question regarding product A04571, anti-ADRA1D antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Parker
Verified customer
Asked: 2016-09-30
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04571 anti-ADRA1D antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-09-30
Question
Will anti-ADRA1D antibody A04571 work on zebrafish IHC with placenta prostate?
H. Wu
Verified customer
Asked: 2016-08-29
Answer
Our lab technicians have not validated anti-ADRA1D antibody A04571 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-ADRA1D antibody A04571 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish placenta prostate in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-08-29
Question
We are currently using anti-ADRA1D antibody A04571 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
L. Li
Verified customer
Asked: 2013-02-20
Answer
The anti-ADRA1D antibody (A04571) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-02-20
