Anti-AFP/Alpha 1 Fetoprotein Antibody (Monoclonal, 35A50)

Afp antibody

Boster Bio Anti-AFP/Alpha 1 Fetoprotein Antibody (Monoclonal, 35A50) catalog # M00522-5. Tested in IHC applications. This antibody reacts with Human.

Product Info Summary

SKU: M00522-5
Size: 100 μg/vial
Reactive Species: Human
Host: Mouse
Application: IHC

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Product Name

Anti-AFP/Alpha 1 Fetoprotein Antibody (Monoclonal, 35A50)

View all Afp Antibodies

SKU/Catalog Number

M00522-5
BM0014 is an alternative SKU for this antibody, used in previous lots.

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-AFP/Alpha 1 Fetoprotein Antibody (Monoclonal, 35A50) catalog # M00522-5. Tested in IHC applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-AFP/Alpha 1 Fetoprotein Antibody (Monoclonal, 35A50) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00522-5)

Host

Mouse

Contents

Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.

Clonality

Monoclonal

Clone Number

35A50

Isotype

Mouse IgG2a

Immunogen

Human alpha-fetoprotein.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

M00522-5 is reactive to Afp in Human

Calculated molecular weight

68.4 kDa

Background of Afp

Alpha-fetoprotein (AFP) is a major plasma protein in the fetus, where it is produced by the yolk sac and liver. Direct confirmation of the assignment of the AFP gene to chromosome 4 by in situ hybridization was provided by Harper and Dugaiczyk (1983), who placed the gene in the q11-q22 region, the same region as the albumin gene. Structure and evolution of human alpha-fetoprotein deduced from partial sequence of cloned cDNA.As the major fetal serum protein, Alpha-fetoprotein is not essential for embryonic development but is required for female fertility.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00522-5 is guaranteed for IHC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 2-4μg/ml, Human

Validation Images & Assay Conditions

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Order DetailsPrice
M00522-5

100μg

$370

Free Secondary Antibody

FREE  $95.00
FREE  $95.00
$40.00  $80.00
$75.00  $150.00
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$67.50  $135.00
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M00522-5
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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This product is for Research Use Only. Not for use in diagnostic procedures.

Update date: Apr 4, 2026