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AGO4 antibody (AZK4IAH1) performs well in IHC and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of Zebrafish AGO4 using anti-Zebrafish AGO4 antibody (AZK4IAH1).
Zebrafish AGO4 was detected in paraffin-embedded section of zebrafish cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Zebrafish AGO4 Antibody (AZK4IAH1) overnight at 4°C.Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Zebrafish AGO4 was detected in paraffin-embedded section of zebrafish cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Zebrafish AGO4 Antibody (AZK4IAH1) overnight at 4°C.Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Zebrafish AGO4 Antibody Picoband®
AGO4 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | IHC |
Tested Applications
AGO4 antibody was positively detected by IHC in:
zebrafish cerebellum tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Zebrafish
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Zebrafish AGO4 Antibody Picoband®
View all AGO4 Antibodies
View all AGO4 Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20 ℃ for one year from date of receipt.
After reconstitution, at 4 ℃ for one month. It can also be aliquotted and stored frozen at -20 ℃ for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived zebrafish AGO4 recombinant protein (Position: K116-L161).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Background of AGO4
AGO4 (Argonaute 4) is also known as EIF2C4. This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic containing PAZ and PIWI domains, and it may play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a cluster of closely related family members including argonaute 3, and eukaryotic translation initiation factor 2C, 1.
Cite This Product
Anti-Zebrafish AGO4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZK4IAH1)
Protocols
Immunohistochemistry (IHC) protocol for AGO4 antibody
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Product Promise
We promise Anti-Zebrafish AGO4 Antibody Picoband® performs excellently in relevant applications for IHC. If it doesn't perform as promised, we will refund the product. Learn more
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AZK4IAH1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026