Product Info Summary
SKU: | A04744-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-AGPAT2 Antibody Picoband™
SKU/Catalog Number
A04744-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-AGPAT2 Antibody Picoband™ catalog # A04744-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-AGPAT2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04744-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human AGPAT2 recombinant protein (Position: R77-N178).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04744-1 is reactive to AGPAT2 in Human, Mouse, Rat
Applications
A04744-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
33 kDa
Calculated molecular weight
30.914kDa
Background of AGPAT2
1-acyl-sn-glycerol-3-phosphate acyltransferase beta is an enzyme that in humans is encoded by the AGPAT2 gene. This gene encodes a member of the 1-acylglycerol-3-phosphate O-acyltransferase family. The protein is located within the endoplasmic reticulum membrane and converts lysophosphatidic acid to phosphatidic acid, the second step in de novo phospholipid biosynthesis. Mutations in this gene have been associated with congenital generalized lipodystrophy (CGL), or Berardinelli-Seip syndrome, a disease characterized by a near absence of adipose tissue and severe insulin resistance. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of AGPAT2 using anti-AGPAT2 antibody (A04744-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: rat liver tissue lysates,
Lane 4: rat pancreas tissue lysates,
Lane 5: mouse liver tissue lysates,
Lane 6: mouse pancreas tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AGPAT2 antigen affinity purified polyclonal antibody (Catalog # A04744-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AGPAT2 at approximately 33 kDa. The expected band size for AGPAT2 is at 33 kDa.
Click image to see more details
Figure 2. IHC analysis of AGPAT2 using anti-AGPAT2 antibody (A04744-1).
AGPAT2 was detected in a paraffin-embedded section of human gall bladder adenosquamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AGPAT2 Antibody (A04744-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of AGPAT2 using anti-AGPAT2 antibody (A04744-1).
AGPAT2 was detected in a paraffin-embedded section of mouse intestines tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AGPAT2 Antibody (A04744-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of AGPAT2 using anti-AGPAT2 antibody (A04744-1).
AGPAT2 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AGPAT2 Antibody (A04744-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For AGPAT2 (Source: Uniprot.org, NCBI)
Gene Name
AGPAT2
Full Name
1-acyl-sn-glycerol-3-phosphate acyltransferase beta
Weight
30.914kDa
Superfamily
1-acyl-sn-glycerol-3-phosphate acyltransferase family
Alternative Names
1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acidacyltransferase, beta); 1-acylglycerol-3-phosphate O-acyltransferase 2; 1-AGPAT 2,1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acidacyltransferase-beta); 1-AGPAT2,1-AGP acyltransferase 2,1-acyl-sn-glycerol-3-phosphate acyltransferase beta; Berardinelli-Seip congenital lipodystrophy; BSCL; BSCL1; EC 2.3.1.51; LPAAB; LPAAT-beta; Lysophosphatidic acid acyltransferase beta; lysophosphatidic acid acyltransferase-beta AGPAT2 1-AGPAT2, BSCL, BSCL1, LPAAB, LPAAT-beta 1-acylglycerol-3-phosphate O-acyltransferase 2 1-acyl-sn-glycerol-3-phosphate acyltransferase beta|1-AGP acyltransferase 2|1-AGPAT 2|1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acid acyltransferase, beta)|lysophosphatidic acid acyltransferase-beta|testicular tissue protein Li 143
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AGPAT2, check out the AGPAT2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AGPAT2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AGPAT2 Antibody Picoband™ (A04744-1)
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