Product Info Summary
| SKU: | A09369-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-AGPAT3 Antibody
SKU/Catalog Number
A09369-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-AGPAT3 Antibody (Catalog# A09369-1). Tested in WB, ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AGPAT3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09369-1)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human AGPAT3. Immunogen sequence location: 121-170
Reactive Species
A09369-1 is reactive to AGPAT3 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
43.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09369-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
ELISA 2000-20000
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
Click image to see more details
Western blot analysis of lysates from K562 cells, using AGPAT3 Antibody. The lane on the right is blocked with the synthesized peptide.
Click image to see more details
Western blot analysis of the lysates from COLO205 cells using AGPAT3 antibody.
Specific Publications For Anti-AGPAT3 Antibody (A09369-1)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-AGPAT3 Antibody
Question
Does anti-AGPAT3 antibody A09369-1 work for WB with melanoma?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
According to the expression profile of melanoma, AGPAT3 is highly expressed in melanoma. So, it is likely that anti-AGPAT3 antibody A09369-1 will work for WB with melanoma.
Boster Scientific Support
Answered: 2020-04-29
Question
We are currently using anti-AGPAT3 antibody A09369-1 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
The anti-AGPAT3 antibody (A09369-1) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-06
Question
Is a blocking peptide available for product anti-AGPAT3 antibody (A09369-1)?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
We do provide the blocking peptide for product anti-AGPAT3 antibody (A09369-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-11-29
Question
Would anti-AGPAT3 antibody A09369-1 work on pig WB with brain?
G. Miller
Verified customer
Asked: 2019-06-03
Answer
Our lab technicians have not tested anti-AGPAT3 antibody A09369-1 on pig. You can run a BLAST between pig and the immunogen sequence of anti-AGPAT3 antibody A09369-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-03
Question
I see that the anti-AGPAT3 antibody A09369-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-07-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-07-03

