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Product Info Summary
| SKU: | M03481 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-AGPS Purified Monoclonal Antibody
SKU/Catalog Number
M03481
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-AGPS Purified Monoclonal Antibody (Catalog# M03481). Tested in Flow Cytometry, WB application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-AGPS Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03481)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
AGPS-03
Isotype
Mouse IgG2a
Immunogen
Recombinant human AGPS (amino acids 158-384). The mouse monoclonal antibody AGPS-03 recognizes AGPS (alkykglycerone phosphate synthase), an intracellular peroxisomal enzyme important for lipid biosynthesis.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M03481 is reactive to AGPS in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
72.9 kDa
Background of AGPS
AGPS (alkylglycerone phosphate synthase), is an enzyme that catalyzes the second step of ether lipid biosynthesis in which acyl-dihydroxyacetone phosphate (acyl-DHAP) is converted to alkyl-DHAP by addition of a long chain alcohol and removal of a long-chain acid anion. The protein is localized to the inner side of the peroxisomal membrane and requires FAD as a cofactor. Mutations in AGPS gene have been associated with type 3 of rhizomelic chondrodysplasia punctata (RCDP3), and Zellweger syndrome. Higher expression of AGPS was observed in BCR/ABL positive leukemias and it was also described to be associated with higher risk of relapse.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03481 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 μg/ml. Intracellular staining.
Western blotting: 1-2 μg/ml.
Validation Images & Assay Conditions
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Separation of K562 cells (red-filled) from human leukocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood spiked with K562 cells stained using anti-AGPS (AGPS-03) purified antibody (concentration in sample 5 µg/ml, GAM APC).
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Flow cytometry intracellular staining pattern of human peripheral whole blood spiked with K562 cells stained using anti-AGPS (AGPS-03) purified antibody (concentration in sample 5 µg/ml, GAM APC).
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Western blotting analysis of human AGPS using mouse monoclonal antibody AGPS-03 on lysates of HEP-G2 and Molt-4 cells, and MCF-7 cells (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-AGPS monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. AGPS was detected at approximately 70 kDa.
Specific Publications For Anti-AGPS Purified Monoclonal Antibody (M03481)
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