Anti-AGRP Antibody Picoband™

AgRP/ART antibody

Boster Bio Anti-AGRP Antibody Picoband™ catalog # A00272-1. Tested in ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: A00272-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IHC

Product Name

Anti-AGRP Antibody Picoband™

View all AgRP/ART Antibodies

SKU/Catalog Number

A00272-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-AGRP Antibody Picoband™ catalog # A00272-1. Tested in ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-AGRP Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00272-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived mouse AGRP recombinant protein (Position: S82-T131).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00272-1 is reactive to Agrp in Human, Mouse, Rat

Applications

A00272-1 is guaranteed for ELISA, IHC Boster Guarantee

Background of AgRP/ART

Agouti-related protein (AgRP), also called agouti-related peptide, is a neuropeptide produced in the brain by the AgRP/NPY neuron. In humans, the agouti-related peptide is encoded by the AGRP gene. This gene encodes an antagonist of the melanocortin-3 and melanocortin-4 receptor. It appears to regulate hypothalamic control of feeding behavior via melanocortin receptor and/or intracellular calcium regulation, and thus plays a role in weight homeostasis. Mutations in this gene have been associated with late on-set obesity.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For Agrp (Source: Uniprot.org, NCBI)

Uniprot ID

P56473

Gene ID

11604

Gene Name

Agrp

Full Name

Agouti-related protein

Weight

14.432kDa

Alternative Names

agouti related protein homolog (mouse); agouti-related protein; AgRP; Agrt; AGRTMGC118963; ART; ARTagouti (mouse) related protein; ASIP2 Agrp|Ag, Agrt, Art|agouti related neuropeptide|agouti-related protein|orexigenic neuropeptide

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Agrp, check out the Agrp Infographic

Agrp infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Agrp: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

A00272-1 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Long-Term l-Serine Administration Reduces Food Intake and Improves Oxidative Stress and Sirt1/NF%u03BAB Signaling in the Hypothalamus of Aging Mice

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3 Customer Q&As for Anti-AGRP Antibody Picoband™

Question

Is a blocking peptide available for product anti-AGRP antibody (A00272-1)?

Verified Customer

Verified customer

Asked: 2019-08-06

Answer

We do provide the blocking peptide for product anti-AGRP antibody (A00272-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-08-06

Question

We are currently using anti-AGRP antibody A00272-1 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-10

Answer

The anti-AGRP antibody (A00272-1) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-10

Question

Can you help my question with product A00272-1, anti-AGRP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

T. Zhang

Verified customer

Asked: 2015-07-01

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00272-1 anti-AGRP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2015-07-01

Size

Conjugation

Total: $315

SKU:A00272-1

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A00272-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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