Anti-Aryl hydrocarbon Receptor/AHR Antibody
Rabbit IgG polyclonal antibody for Aryl hydrocarbon receptor(AHR) detection. Tested with WB, IHC-P in Mouse.
Anti-Aryl hydrocarbon Receptor/AHR Antibody Info At A Glance
|Product Name||Anti-Aryl hydrocarbon Receptor/AHR Antibody
See all Ahr primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for AHR detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Mouse. AHR information: Molecular Weight: 95017 MW; Subcellular Localization: Cytoplasm. Nucleus. Initially cytoplasmic; upon binding with ligand and interaction with a HSP90, it translocates to the nucleus; Tissue Specificity: Expressed in all tissues tested including brain, heart, kidney, liver, lung, muscle, ovary, skin, spleen and thymus.|
|Cite This Product||Anti-Aryl hydrocarbon Receptor/AHR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1782-2)|
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*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of mouse AHR (497-511aa EAALKHEQIGHAQDV).|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For Ahr (Source: Uniprot.org, NCBI)
|NCBI Gene Id||11622|
|Species Of This Entry||Mouse|
|Protein Name||Aryl hydrocarbon receptor|
|Alternative Names||Ahr|Ah receptor; AHR; AH-receptor; aryl hydrocarbon receptor; BHLHE76; bHLHe76aromatic hydrocarbon receptor; Class E basic helix-loop-helix protein 76|
|Post Tranlational Modifications||Phosphorylation; Methylation; Oxidation; Hydroxylation; Acetylation; Cleavage; Reduction; Ubiquitination; Demethylation; Dephosphorylation; Bromination; Sumoylation; Nitration; Glycosylation; Glutathionylation; Phosphorothioation; Deamination; Deglutathionylation; Glutathionation; Myristoylation|
|Gene Location||12 A3|12 15.78 cM, on Chromasome 12, gene sequence:|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Ahr (Source: Uniprot.org, NCBI)
|Protein Function||Ligand-activated transcriptional activator. Binds to the XRE promoter region of genes it activates. Activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. Involved in cell-cycle regulation. Likely to play an important role in the development and maturation of many tissues. Regulates the circadian clock by inhibiting the basal and circadian expression of the core circadian component PER1. Inhibits PER1 by repressing the CLOCK-ARNTL/BMAL1 heterodimer mediated transcriptional activation of PER1. The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed:28396409).|
|Tissue Specificity||Expressed in all tissues tested including brain, heart, kidney, liver, lung, muscle, ovary, skin, spleen and thymus.|
|Research Areas||Apoptosis; Breast Cancer; Cancer; Chromatin Research; Hypoxia; Transcription Factors and Regulators|
|Related Diseases||Asthma; Inflammation; Bronchial Hyperreactivity; Malignant Neoplasms; Liver Carcinoma; Neoplasms; Eosinophilia; Pneumonia; Malignant Neoplasm Of Breast; Allergic Asthma; Respiratory Hypersensitivity; Carcinogenesis; Mammary Neoplasms; Allergy; Hyperplasia; Carcinoma; Liver Neoplasms; Lung Diseases; Airway Disease; Inflammatory Response|
|Related Pathways||Sensitization; Pathogenesis; Cell Cycle; Cell Proliferation; Localization; Secretion; Cytokine Production; Inflammatory Response; Immune Response; Cell Growth; Transport; Cell Death; Cell Differentiation; Cell Activation; Hypersensitivity; Methylation; Translation; Rna Interference; Cell Cycle Arrest; Cell Adhesion|
|Background||AHR(aryl hydrocarbon receptor), also called bHLHe76, is a member of the family of basic helix-loop-helix transcription factors. AhR is a cytosolic transcription factor that is normally inactive, bound to several co-chaperones. The AHR gene is mapped on 7p21.1. Estrogenic actions of AHR agonists were detected in wildtype ovariectomized mouse uteri, but were absent in Ahr -/- or Er-alpha -/- ovariectomized mice. Complex assembly and ubiquitin ligase activity of CUL4B(AHR) in vitro and in vivo are dependent on the AHR ligand. In the CUL4B(AHR) complex, ligand-activated AHR acts as a substrate-specific adaptor component that targets sex steroid receptors for degradation. Cd4-positive cells from mice lacking Ahr developed Th17 responses but failed to produce Il22 and did not show enhanced Th17 development. Activation of Ahr during induction of EAE accelerated disease onset and increased pathology in wildtype mice, but not in Ahr -/- mice. The TDO-AHR pathway is active in human brain tumors and is associated with malignant progression and poor survival. Ahr activity within ROR-gamma-t-positive ILC could be induced by dietary ligands such as those contained in vegetables of the family Brassicaceae.|
|Scientific References||PMID: 1314586 by Ema M., et al. cDNA cloning and structure of mouse putative Ah receptor.|
PMID: 1325649 by Burbach K.M., et al. Cloning of the Ah-receptor cDNA reveals a distinctive ligand- activated transcription factor.
Our Boster Quality Guarantee for Anti-Aryl hydrocarbon Receptor/AHR Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: Mouse Brain Tissue Lysate
Lane 2: Mouse Heart Tissue Lysate
Lane 3: Mouse Liver Tissue Lysate
IHC(P): Mouse Cardiac Muscle Tissue
IHC(P): Mouse Brain Tissue
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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Note: sample size takes longer to process, average 5-7 days.
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