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Product Info Summary
| SKU: | M00225 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-AhR Rabbit Monoclonal Antibody
SKU/Catalog Number
M00225
BM4965 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-AhR Rabbit Monoclonal Antibody catalog # M00225. Tested in WB, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AhR Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00225)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AOHI-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human AhR
Reactive Species
M00225 is reactive to AHR in Human
Observed Molecular Weight
96,105 kDa
Calculated molecular weight
96.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00225 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
Positive Control
WB: human RT4 whole cell, human U-87MG whole cell
Validation Images & Assay Conditions
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Western blot analysis of AhR using anti-AhR antibody (M00225).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human U-87MG whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AhR antigen affinity purified monoclonal antibody (Catalog # M00225) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AhR at approximately 96,105 kDa. The expected band size for AhR is at 96 kDa.
Specific Publications For Anti-AhR Rabbit Monoclonal Antibody (M00225)
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4 Customer Q&As for Anti-AhR Rabbit Monoclonal Antibody
Question
We are currently using anti-AhR Rabbit Monoclonal antibody M00225 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
The anti-AhR Rabbit Monoclonal antibody (M00225) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-16
Question
We purchased anti-AhR Rabbit Monoclonal antibody for WB on placenta last year. I am using human, and We intend to use the antibody for IF next. I am looking for examining placenta as well as liver in our next experiment. Could give a recommendation on which antibody would work the best for IF?
G. Gonzalez
Verified customer
Asked: 2019-03-28
Answer
I looked at the website and datasheets of our anti-AhR Rabbit Monoclonal antibody and I see that M00225 has been tested on human in both WB and IF. Thus M00225 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-03-28
Question
We have observed staining in human liver. Do you have any suggestions? Is anti-AhR Rabbit Monoclonal antibody supposed to stain liver positively?
T. Wu
Verified customer
Asked: 2018-04-30
Answer
From literature liver does express AHR. From Uniprot.org, AHR is expressed in placenta, liver, among other tissues. Regarding which tissues have AHR expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 8393992
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-04-30
Question
My team were happy with the WB result of your anti-AhR Rabbit Monoclonal antibody. However we have seen positive staining in placenta cytoplasm. using this antibody. Is that expected? Could you tell me where is AHR supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-06-01
Answer
From literature, placenta does express AHR. Generally AHR expresses in cytoplasm. Regarding which tissues have AHR expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 8393992
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-06-01
