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Product Info Summary
| SKU: | A01515-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-AHRR Antibody Picoband™
SKU/Catalog Number
A01515-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-AHRR Antibody Picoband™ catalog # A01515-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-AHRR Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01515-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human AHRR recombinant protein (Position: L108-D180).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01515-2 is reactive to Ahrr in Human, Mouse
Applications
A01515-2 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
76 kDa
Calculated molecular weight
77.726kDa
Background of Ahrr
The aryl-hydrocarbon receptor repressor also known as AHRR is a human gene. The protein encoded by this gene participates in the aryl hydrocarbon receptor (AhR) signaling cascade, which mediates dioxin toxicity, and is involved in regulation of cell growth and differentiation. It functions as a feedback modulator by repressing AhR-dependent gene expression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of AHRR using anti-AHRR antibody (A01515-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AHRR antigen affinity purified polyclonal antibody (Catalog # A01515-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AHRR at approximately 76 kDa. The expected band size for AHRR is at 76 kDa.
Protein Target Info & Infographic
Gene/Protein Information For Ahrr (Source: Uniprot.org, NCBI)
Gene Name
Ahrr
Full Name
Aryl hydrocarbon receptor repressor
Weight
77.726kDa
Alternative Names
Aryl hydrocarbon receptor repressor Ahrr|mKIAA1234|aryl-hydrocarbon receptor repressor|aryl hydrocarbon receptor repressor|ahR repressor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ahrr, check out the Ahrr Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ahrr: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AHRR Antibody Picoband™ (A01515-2)
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No publications found for A01515-2
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