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Product Info Summary
| SKU: | A01795 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-AIM CD5L Antibody
SKU/Catalog Number
A01795
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-AIM CD5L Antibody (Catalog # A01795). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse.
Storage & Handling
AIM antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-AIM CD5L Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01795)
Host
Rabbit
Contents
AIM Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
AIM antibody was raised against a 13 amino acid synthetic peptide near the carboxy terminus of human AIM. The immunogen is located within amino acids 250 - 300 of AIM.
Reactive Species
A01795 is reactive to CD5L in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
38.1 kDa
Background of CD5L
Apoptosis inhibitor of macrophages (AIM) is a member of the scavenger receptor cysteine-rich domain superfamily (SRCR-SF) initially identified as an inducible cell surface ligand of CD5. It was shown that AIM functions in the thymus as the inducer of resistance to apoptosis within CD4+/CD8+ thymocytes and as the supporter of the viability of these cells before thymic selection. AIM was also shown to support macrophage survival and enhance their phagocytic function. More recent experiments using recombinant AIM significantly inhibited apoptosis of NKT and T cells obtained from C. parvum-stimulated livers in vitro, suggesting that AIM functions to induce resistance to apoptosis in these cells and supports host defense against inflammation during infection.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01795 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
AIM antibody can be used for the detection of AIM by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of AIM in Raji lysate with AIM antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
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Immunohistochemistry of AIM in human lymph node tissue with AIM antibody at 10 μg/mL.
Specific Publications For Anti-AIM CD5L Antibody (A01795)
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