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Product Info Summary
| SKU: | A09025 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Adenylate kinase isoenzyme 6 AK6 Antibody
SKU/Catalog Number
A09025
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Adenylate kinase isoenzyme 6 AK6 Antibody catalog # A09025. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Adenylate kinase isoenzyme 6 AK6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09025)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human KAD6. AA range:11-60
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A09025 is reactive to AK6 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
20.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09025 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using AK6 Polyclonal Antibody
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Western blot analysis of lysates from Jurkat cells, using KAD6 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4° overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Specific Publications For Anti-Adenylate kinase isoenzyme 6 AK6 Antibody (A09025)
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Customer Q&As
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5 Customer Q&As for Anti-Adenylate kinase isoenzyme 6 AK6 Antibody
Question
Does anti-AK6 antibody A09025 work for WB with lymph?
Verified Customer
Verified customer
Asked: 2018-06-29
Answer
According to the expression profile of lymph, AK6 is highly expressed in lymph. So, it is likely that anti-AK6 antibody A09025 will work for WB with lymph.
Boster Scientific Support
Answered: 2018-06-29
Question
My question regarding product A09025, anti-AK6 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
N. Wu
Verified customer
Asked: 2016-06-13
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A09025 anti-AK6 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-06-13
Question
We are currently using anti-AK6 antibody A09025 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
R. Li
Verified customer
Asked: 2014-11-13
Answer
The anti-AK6 antibody (A09025) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-11-13
Question
My question regards to test anti-AK6 antibody A09025 on mouse lymph for research purposes, then I may be interested in using anti-AK6 antibody A09025 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Williams
Verified customer
Asked: 2014-08-05
Answer
The products we sell, including anti-AK6 antibody A09025, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-08-05
Question
Is a blocking peptide available for product anti-AK6 antibody (A09025)?
C. Anderson
Verified customer
Asked: 2013-05-21
Answer
We do provide the blocking peptide for product anti-AK6 antibody (A09025). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2013-05-21
