Anti-AKAP1 Antibody

Boster Bio Anti-AKAP1 Antibody catalog # A04451. Tested in IF, IHC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A04451
Size: 100μl
Reactive Species: Human
Host: Rabbit
Application: IF, IHC, WB

Product Name

Anti-AKAP1 Antibody

See all AKAP1 products

SKU/Catalog Number

A04451

Size

100μl

Form

Liquid

Description

Boster Bio Anti-AKAP1 Antibody catalog # A04451. Tested in IF, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-AKAP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04451)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Immunogen

Synthesized peptide derived from internal of human AKAP1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04451 is reactive to AKAP1 in Human

Applications

A04451 is guaranteed for IF, IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:3000
IHC, 1:50-1:100
Immunofluorescence, 1:100-1:500

Validation Images & Assay Conditions

Gene/Protein Information For AKAP1 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q92667

Gene ID

8165

Gene Name

AKAP1

Full Name

A-kinase anchor protein 1, mitochondrial

Weight

97.342kDa

Alternative Names

A kinase (PRKA) anchor protein 1; a kinase anchor protein 1, mitochondrial; AKAP121; AKAP149AKAP 149; AKAP84; A-kinase anchor protein 1, mitochondrial; A-kinase anchor protein 149 kDa; A-kinase anchor protein, 149kD; D-AKAP1; D-AKAP-1; Dual specificity A-kinase-anchoring protein 1; dual-specificity A-kinase anchoring protein 1; MGC1807; PRKA1; protein kinase A anchoring protein 1; protein kinase A1; Protein kinase A-anchoring protein 1; protein kinase anchoring protein 1; S-AKAP84; SAKAP84AKAP; Spermatid A-kinase anchor protein 84 AKAP1 AKAP21, AKAP149, AKAP84, D-AKAP1, PPP1R43, PRKA1, SAKAP84, TDRD17, AKAP1 A-kinase anchoring protein 1 A-kinase anchor protein 1, mitochondrial|A kinase (PRKA) anchor protein 1|A-kinase anchor protein 149 kDa|AKAP 149|D-AKAP-1|dual-specificity A-kinase anchoring protein 1|protein kinase A anchoring protein 1|protein kinase A1|protein phosphatase 1, regulatory subunit 43|spermatid A-kinase anchor protein 84|testicular secretory protein Li 5|tudor domain containing 17

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on AKAP1, check out the AKAP1 Infographic

AKAP1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for AKAP1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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13 Customer Q&As for Anti-AKAP1 Antibody

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-AKAP1 antibody A04451. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-05-07

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-05-07

Question

Would anti-AKAP1 antibody A04451 work on feline WB with cervix carcinoma?

Verified Customer

Verified customer

Asked: 2020-04-28

Answer

Our lab technicians have not tested anti-AKAP1 antibody A04451 on feline. You can run a BLAST between feline and the immunogen sequence of anti-AKAP1 antibody A04451 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline cervix carcinoma in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-04-28

Question

My question regarding product A04451, anti-AKAP1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-04-17

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04451 anti-AKAP1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-04-17

Question

Does anti-AKAP1 antibody A04451 work for IF with cervix carcinoma erythroleukemia?

Verified Customer

Verified customer

Asked: 2020-02-28

Answer

According to the expression profile of cervix carcinoma erythroleukemia, AKAP1 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-AKAP1 antibody A04451 will work for IF with cervix carcinoma erythroleukemia.

Boster Scientific Support

Answered: 2020-02-28

Question

I see that the anti-AKAP1 antibody A04451 works with IF, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-02-12

Answer

You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-02-12

Question

Is a blocking peptide available for product anti-AKAP1 antibody (A04451)?

Verified Customer

Verified customer

Asked: 2020-01-13

Answer

We do provide the blocking peptide for product anti-AKAP1 antibody (A04451). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-01-13

Question

I was wanting to use your anti-AKAP1 antibody for IF for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?

T. Jackson

Verified customer

Asked: 2019-06-14

Answer

You can see on the product datasheet, A04451 anti-AKAP1 antibody has been validated for IF, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-06-14

Question

Here is the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-AKAP1 antibody A04451. Please let me know if you require anything else.

D. Parker

Verified customer

Asked: 2019-01-18

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-01-18

Question

Do you have a BSA free version of anti-AKAP1 antibody A04451 available?

G. Rodriguez

Verified customer

Asked: 2017-01-18

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-AKAP1 antibody A04451 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-01-18

Question

I was wanting to use to test anti-AKAP1 antibody A04451 on human cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-AKAP1 antibody A04451 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

K. Singh

Verified customer

Asked: 2014-04-08

Answer

The products we sell, including anti-AKAP1 antibody A04451, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2014-04-08

Question

Will A04451 anti-AKAP1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

M. Kulkarni

Verified customer

Asked: 2014-03-21

Answer

As indicated on the product datasheet, A04451 anti-AKAP1 antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2014-03-21

Question

We are currently using anti-AKAP1 antibody A04451 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?

K. Brown

Verified customer

Asked: 2013-05-10

Answer

The anti-AKAP1 antibody (A04451) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-05-10

Question

Is this A04451 anti-AKAP1 antibody reactive to the isotypes of AKAP1?

J. Anderson

Verified customer

Asked: 2013-03-06

Answer

The immunogen of A04451 anti-AKAP1 antibody is Synthesized peptide derived from internal of human AKAP1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2013-03-06

Size

Total: $299

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
A04451
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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