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Product Info Summary
| SKU: | M15886 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-AKAP14 Rabbit Monoclonal Antibody
SKU/Catalog Number
M15886
BM4536 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-AKAP14 Rabbit Monoclonal Antibody catalog # M15886. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AKAP14 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M15886)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
FGO-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human AKAP14
Reactive Species
M15886 is reactive to AKAP14 in Human, Mouse, Rat
Observed Molecular Weight
21,23 kDa
Calculated molecular weight
22.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M15886 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
IP 1:20
Positive Control
WB: human Jurkat whole cell, human A549 whole cell, human HepG2 whole cell, human RT4 whole cell, rat testis tissue, rat C6 whole cell, mouse testis tissue, mouse Neuro-2a whole cell
Validation Images & Assay Conditions
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Western blot analysis of AKAP14 using anti-AKAP14 antibody (M15886).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human RT4 whole cell lysates,
Lane 5: rat testis tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse testis tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKAP14 antigen affinity purified monoclonal antibody (Catalog # M15886) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKAP14 at approximately 21,23 kDa. The expected band size for AKAP14 is at 21 kDa.
Specific Publications For Anti-AKAP14 Rabbit Monoclonal Antibody (M15886)
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1 Customer Q&As for Anti-AKAP14 Rabbit Monoclonal Antibody
Question
We are currently using anti-AKAP14 Rabbit Monoclonal antibody M15886 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
The anti-AKAP14 Rabbit Monoclonal antibody (M15886) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-18
