Product Info Summary
SKU: | A13113 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-AKR1CL2 AKR1E2 Antibody
SKU/Catalog Number
A13113
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-AKR1CL2 AKR1E2 Antibody (Catalog # A13113). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AKR1CL2 AKR1E2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13113)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Immunogen
Synthesized peptide derived from internal of human AKR1CL2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A13113 is reactive to AKR1E2 in Human
Applications
A13113 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
36.589kDa
Background of AKR1CL2
Catalyzes the NADPH-dependent reduction of 1,5-anhydro-D-fructose (AF) to 1,5-anhydro-D-glucitol. Can also catalyze the reduction of various aldehydes and quinones By similarity. Has low NADPH-dependent reductase activity towards 9,10-phenanthrenequinone (in vitro).
Nishinaka T., Chem. Biol. Interact. 143:299-305(2003).
Hyndman D.J., Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from HepG2 cells and Jurkat cells, using AKR1CL2 antibody A13113.
Protein Target Info & Infographic
Gene/Protein Information For AKR1E2 (Source: Uniprot.org, NCBI)
Gene Name
AKR1E2
Full Name
1,5-anhydro-D-fructose reductase
Weight
36.589kDa
Superfamily
aldo/keto reductase family
Alternative Names
AF reductase; AKR1CL2; AKRDC1; Aldo-keto reductase family 1 member C-like protein 21,5-anhydro-D-fructose reductase; Aldo-keto reductase family 1 member E2; aldo-keto reductase family 1, member C-like 2; aldo-keto reductase family 1, member E2; aldo-keto reductase loopADR; aldo-keto reductase related protein; EC 1.1.1; EC 1.1.1.263; hTSP; LoopADR; MGC10612; Testis-specific protein AKR1E2 AKR1CL2, AKRDC1, HTSP1, LoopADR, TAKR, hTSP, htAKR aldo-keto reductase family 1 member E2 1,5-anhydro-D-fructose reductase|AF reductase|aldo-keto reductase family 1 member C-like protein 2|aldo-keto reductase family 1, member C-like 2|aldo-keto reductase loopADR|human testis aldo-keto reductase|human testis specific protein|testis aldo-keto reductase|testis-specific protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AKR1E2, check out the AKR1E2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AKR1E2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AKR1CL2 AKR1E2 Antibody (A13113)
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No publications found for A13113
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-AKR1CL2 AKR1E2 Antibody
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-AKR1CL2 antibody A13113. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-30
Question
We are currently using anti-AKR1CL2 antibody A13113 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
The anti-AKR1CL2 antibody (A13113) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-02
Question
I was wanting to use your anti-AKR1CL2 antibody for WB for human testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human testis identification?
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
As indicated on the product datasheet, A13113 anti-AKR1CL2 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-15