Anti-Akt (E17K Mutant) AKT1 Rabbit Monoclonal Antibody, Clone#RM336

Akt1 antibody

Boster Bio Anti-Akt (E17K Mutant) AKT1 Rabbit Monoclonal Antibody, Clone#RM336 (Catalog # M00024-2). Tested in WB, ELISA, IHC applications. This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: M00024-2
Size: 50ug
Reactive Species: Human
Host: Rabbit
Application: ELISA, IHC, WB

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Product Name

Anti-Akt (E17K Mutant) AKT1 Rabbit Monoclonal Antibody, Clone#RM336

View all Akt1 Antibodies

SKU/Catalog Number

M00024-2

Size

50ug

Form

Liquid

Description

Boster Bio Anti-Akt (E17K Mutant) AKT1 Rabbit Monoclonal Antibody, Clone#RM336 (Catalog # M00024-2). Tested in WB, ELISA, IHC applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Akt (E17K Mutant) AKT1 Rabbit Monoclonal Antibody, Clone#RM336 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00024-2)

Host

Rabbit

Contents

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Clonality

Monoclonal

Clone Number

RM336

Isotype

Rabbit IgG

Immunogen

A peptide corresponding to the Akt E17K Mutant

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody reacts to the Akt E17K mutant. No cross-reactivity with wild type Akt.

Reactive Species

M00024-2 is reactive to AKT1 in Human

Applications

M00024-2 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

55.686kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 0.1 ug/mL – 0.5 ug/mL
ELISA: 0.05 ug/mL - 0.5 ug/mL
Immunohistochemistry: 0.2 – 1 ug/mL.

Validation Images & Assay Conditions

Gene/Protein Information For AKT1 (Source: Uniprot.org, NCBI)

Gene Name

AKT1

Full Name

RAC-alpha serine/threonine-protein kinase

Weight

55.686kDa

Superfamily

protein kinase superfamily

Alternative Names

AKT serine/threonine kinase 1; AKT; Akt1; AKT1m; CWS6; EC 2.7.11; EC 2.7.11.1; PKB alpha; PKB; PKBMGC99656; PRKBA; Protein Kinase B Alpha; Protein kinase B; Proto-oncogene c-Akt; rac protein kinase alpha; RAC; RAC-alpha serine/threonine-protein kinase; RAC-alpha; RAC-PK-alpha; RACPKB-ALPHA; Serine-Threonine Protein Kinase ; v-akt murine thymoma viral oncogene homolog 1; V-Akt Murine Thymoma Viral Oncogene-Like Protein 1 AKT1 AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA AKT serine/threonine kinase 1 RAC-alpha serine/threonine-protein kinase|AKT1m|PKB alpha|RAC-PK-alpha|protein kinase B alpha|proto-oncogene c-Akt|rac protein kinase alpha|serine-threonine protein kinase|v-akt murine thymoma viral oncogene homolog 1|v-akt murine thymoma viral oncogene-like protein 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on AKT1, check out the AKT1 Infographic

AKT1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for AKT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00024-2 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

A small interfering RNA targeting osteopontin as gastric cancer therapeutics

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5 Customer Q&As for Anti-Akt (E17K Mutant) AKT1 Rabbit Monoclonal Antibody, Clone#RM336

Question

We are currently using anti-Akt (E17K Mutant) Rabbit Monoclonal antibody, Clone#RM336 M00024-2 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-23

Answer

The anti-Akt (E17K Mutant) Rabbit Monoclonal antibody, Clone#RM336 (M00024-2) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-23

Question

We have observed staining in human cervix carcinoma erythroleukemia. Are there any suggestions? Is anti-Akt (E17K Mutant) Rabbit Monoclonal antibody, Clone#RM336 supposed to stain cervix carcinoma erythroleukemia positively?

Verified Customer

Verified customer

Asked: 2020-03-18

Answer

From what I have seen in literature cervix carcinoma erythroleukemia does express AKT1. From what I have seen in Uniprot.org, AKT1 is expressed in left adrenal gland, adrenal gland, muscle ovary, foreskin, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have AKT1 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Foreskin, Pubmed ID: 1718748
Liver, Pubmed ID: 24275569
Muscle, and Ovary, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-03-18

Question

We bought anti-Akt (E17K Mutant) Rabbit Monoclonal antibody, Clone

Verified Customer

Verified customer

Asked: 2019-12-11

Answer

I have checked the website and datasheets of our anti-Akt (E17K Mutant) Rabbit Monoclonal antibody, Clone

Boster Scientific Support

Answered: 2019-12-11

Question

I am looking for using your anti-Akt (E17K Mutant) Rabbit Monoclonal antibody, Clone#RM336 for regulation of tp53 activity through acetylation studies. Has this antibody been tested with western blotting on 293t cells? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2017-11-27

Answer

We appreciate your inquiry. This M00024-2 anti-Akt (E17K Mutant) Rabbit Monoclonal antibody, Clone#RM336 is tested on 293t cells. It is guaranteed to work for ELISA, IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-11-27

Question

Our lab were content with the WB result of your anti-Akt (E17K Mutant) Rabbit Monoclonal antibody, Clone

Verified Customer

Verified customer

Asked: 2017-06-23

Answer

Based on literature, muscle ovary does express AKT1. Generally AKT1 expresses in cytoplasm. Regarding which tissues have AKT1 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Foreskin, Pubmed ID: 1718748
Liver, Pubmed ID: 24275569
Muscle, and Ovary, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2017-06-23

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M00024-2
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