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Product Info Summary
| SKU: | M00024-2 |
|---|---|
| Size: | 50ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Akt (E17K Mutant) AKT1 Rabbit Monoclonal Antibody, Clone#RM336
SKU/Catalog Number
M00024-2
Size
50ug
Form
Liquid
Description
Boster Bio Anti-Akt (E17K Mutant) AKT1 Rabbit Monoclonal Antibody, Clone#RM336 (Catalog # M00024-2). Tested in WB, ELISA, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Akt (E17K Mutant) AKT1 Rabbit Monoclonal Antibody, Clone#RM336 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00024-2)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM336
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the Akt E17K Mutant
Cross-reactivity
This antibody reacts to the Akt E17K mutant. No cross-reactivity with wild type Akt.
Reactive Species
M00024-2 is reactive to AKT1 in Human
Calculated molecular weight
55.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00024-2 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 0.1 ug/mL – 0.5 ug/mL
ELISA: 0.05 ug/mL - 0.5 ug/mL
Immunohistochemistry: 0.2 – 1 ug/mL.
Validation Images & Assay Conditions
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Western Blotting result
Western Blot analysis of a cell lysate mixture of untransfected 293T and 293T transfected with a DNA construct encoding the Akt E17K mutant, using anti-Akt E17K (clone RM336) (0.1ug/mL) or anti-Akt (clone RM252) rabbit monoclonal antibody.
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IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded 293T cells transfected with a DNA construct encoding the Akt E17K mutation or Histone H3.3, stained with anti-Akt E17K rabbit monoclonal antibody (clone RM336) at 0.2ug/mL.
Specific Publications For Anti-Akt (E17K Mutant) AKT1 Rabbit Monoclonal Antibody, Clone#RM336 (M00024-2)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Akt (E17K Mutant) AKT1 Rabbit Monoclonal Antibody, Clone#RM336
Question
We are currently using anti-Akt (E17K Mutant) Rabbit Monoclonal antibody, Clone#RM336 M00024-2 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
The anti-Akt (E17K Mutant) Rabbit Monoclonal antibody, Clone#RM336 (M00024-2) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-23
Question
We have observed staining in human cervix carcinoma erythroleukemia. Are there any suggestions? Is anti-Akt (E17K Mutant) Rabbit Monoclonal antibody, Clone#RM336 supposed to stain cervix carcinoma erythroleukemia positively?
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
From what I have seen in literature cervix carcinoma erythroleukemia does express AKT1. From what I have seen in Uniprot.org, AKT1 is expressed in left adrenal gland, adrenal gland, muscle ovary, foreskin, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have AKT1 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Foreskin, Pubmed ID: 1718748
Liver, Pubmed ID: 24275569
Muscle, and Ovary, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-03-18
Question
We bought anti-Akt (E17K Mutant) Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
I have checked the website and datasheets of our anti-Akt (E17K Mutant) Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2019-12-11
Question
I am looking for using your anti-Akt (E17K Mutant) Rabbit Monoclonal antibody, Clone#RM336 for regulation of tp53 activity through acetylation studies. Has this antibody been tested with western blotting on 293t cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-11-27
Answer
We appreciate your inquiry. This M00024-2 anti-Akt (E17K Mutant) Rabbit Monoclonal antibody, Clone#RM336 is tested on 293t cells. It is guaranteed to work for ELISA, IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-11-27
Question
Our lab were content with the WB result of your anti-Akt (E17K Mutant) Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2017-06-23
Answer
Based on literature, muscle ovary does express AKT1. Generally AKT1 expresses in cytoplasm. Regarding which tissues have AKT1 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Foreskin, Pubmed ID: 1718748
Liver, Pubmed ID: 24275569
Muscle, and Ovary, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-06-23
