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AKT1 antibody (P00024-4) performs well in IP, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 22 Publications

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Western blot analysis of Phospho-AKT1 (S124) expression in (1) MCF-7 cell treated with Alkaline Phosphatase lysate; (2) MCF-7 cell lysate.

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Anti-Phospho-AKT1 (S124) Rabbit Monoclonal Antibody

Name: Anti-Phospho-AKT1 (S124) Rabbit Monoclonal Antibody
Brand: Boster Bio
SKU: P00024-4
Price: 370 USD
Availability: InStock
Rating: 100 (1 reviews)

AKT1 antibody

22 Publications|

1 Review

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Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	IP, WB

Product Information

Product Name

Anti-Phospho-AKT1 (S124) Rabbit Monoclonal Antibody
View all AKT1 Antibodies

Form

Liquid

Uniprot

P31749

Gene ID

207

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

HIA-1

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human AKT1

Observed Molecular Weight

60 kDa

Calculated Molecular Weight

55.7 kDa

Cite This Product

Anti-Phospho-AKT1 (S124) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00024-4)

Publications

Rational design of curcumin-borneol hybrids for rheumatoid arthritis pathological intervention via AKT/mTOR/HIF-1α pathway modulation

Published: 2026-05-11

Journal: MATERIALS & DESIGN

Application: WB

Impact Factor: 8.20

Species: Rat

SKU	P00024-4
Application	Western Blot
Sample	mouse 4T1 cells
Sample Processing Description	normal 4T1 cells, LPS-stimulated 4T1 cells, cells treated with low, medium, or high doses of the drug, and cells treated with a positive control drug.
Other Reagents	RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody	Anti-AKT1,2,3 Antibody Picoband®
Primary Incubation	1:10000, overnight at 4 ℃
Secondary Antibody	HRP Goat Anti-Rabbit IgG
Secondary Incubation	1:10000, 1 hour in room temperature
Detection	Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary	Under equal loading conditions, the total AKT protein levels were similar across all groups, while the amount of phosphorylated AKT1 showed significant changes. The blank group had the lowest level, the model group had the highest, and in the drug-treated groups, the levels decreased progressively from low to medium to high dose. The positive control drug group was close to the high-dose group.

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Free Secondary Antibody

Anti-Phospho-AKT1 (S124) Rabbit Monoclonal Antibody

AKT1 antibody

Overview

Recommend Dilution

Product Information

Publications

Rational design of curcumin-borneol hybrids for rheumatoid arthritis pathological intervention via AKT/mTOR/HIF-1α pathway modulation

ApoE-T807-modified tanshinone IIA liposomes enhance cognitive function in APP/PS1 mice by inhibiting Tau phosphorylation via the PI3K/AKT signaling pathway

Black phosphorus quantum dots induce lipid accumulation through PPARγ activation and mitochondrial dysfunction in adipocytes

The E3 ubiquitin ligase FBXO38 maintains the antitumor function of natural killer cells by sustaining IL-15R signaling

circMTO1/miR-30c-5p/SOCS3 axis alleviates oral submucous fibrosis through inhibiting fibroblast–myofibroblast transition

Pungent agents derived from the fruits of Zanthoxylum armatum DC. are beneficial for ameliorating type 2 diabetes mellitus via regulation of AMPK/PI3K/Akt signaling

Rapamycin Regulates Lipopolysaccharide-Induced Microglial Phagocytosis In Vitro

The role of phosphoprotein associated with glycosphingolipid-enriched microdomains 1 (PAG1) in regulating the progression of oral squamous cell carcinoma

Mechanism investigation of Duhuo Jisheng pill against rheumatoid arthritis based on a strategy for the integration of network pharmacology, molecular docking and in vivo experimental verification

A prognostic biomarker NRG1 promotes U‑87 MG glioblastoma cell malignancy by inhibiting autophagy via ERBB2/AKT/mTOR pathway

Triptolide inhibits the Apoptosis and Reactive Oxygen Species Production in Hydrogen Peroxide-Treated Nasal Epithelial Cells by Regulating PTEN/PI3K/AKT Signaling Pathway

Integration of pharmacodynamics and metabolomics reveals the therapeutic effects of 6-acetylacteoside on ovariectomy-induced osteoporosis mice

Exosomes derived from PC‑3 cells suppress osteoclast differentiation by downregulating miR‑148a and blocking the PI3K/AKT/mTOR pathway

Anti-Myocardial Ischemia Reperfusion Injury Mechanism of Dried Ginger-Aconite Decoction Based on Network Pharmacology

Mechanism of Action of Xiaoyao San in Treatment of Ischemic Stroke is Related to Anti-Apoptosis and Activation of PI3K/Akt Pathway

Mechanisms underlying reproductive toxicity induced by nickel nanoparticles identified by comprehensive gene expression analysis in GC-1 spg cells

Effect and mechanism of PI3K/AKT/mTOR signaling pathway in the apoptosis of GC-1 cells induced by nickel nanoparticles

Comprehensive circular RNA profiling reveals the regulatory role of circRNA_0007694 in papillary thyroid carcinoma

LC-MS analysis of Myrica rubra extract and its hypotensive effects via the inhibition of GLUT 1 and activation of the NO/Akt/eNOS signaling pathway

miR-21 promotes proliferation and inhibits apoptosis of hepatic stellate cells through targeting PTEN/PI3K/AKT pathway

The impaction of clock-knockdown on autophagy in 4T1 breast cancer cells

Bone marrow mesenchymal stem cell-derived exosomal miR-21 protects C-kit+ cardiac stem cells from oxidative injury through the PTEN/PI3K/Akt axis

Review

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1 Review For Anti-Phospho-AKT1 (S124) Rabbit Monoclonal Antibody

The antibody shows a clear target, correct localization, minimal background, and overall good results.

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