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Product Info Summary
| SKU: | P00024-4 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-Phospho-AKT1 (S124) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00024-4
BM4762 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-AKT1 (S124) Rabbit Monoclonal Antibody catalog # P00024-4. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-AKT1 (S124) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00024-4)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
HIA-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human AKT1
Reactive Species
P00024-4 is reactive to AKT1 in Human, Mouse, Rat
Observed Molecular Weight
60 kDa
Calculated molecular weight
55.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00024-4 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IP 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of Phospho-AKT1 (S124) expression in (1) MCF-7 cell treated with Alkaline Phosphatase lysate; (2) MCF-7 cell lysate.
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Western blot analysis of Phospho-AKT1 (S124) using anti-Phospho-AKT1 (S124) antibody (P00024-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse 4T1 whole cell lysates,
Lane 2: LPS-stimulated mouse 4T1 whole cell lysates,
Lane 3: Low-dose drug mouse 4T1 whole cell lysates,
Lane 4: Medium-dose drug mouse 4T1 whole cell lysates,
Lane 5: High-dose drug mouse 4T1 whole cell lysates,
Lane 6: Positive control drug mouse 4T1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Phospho-AKT1 (S124) antigen affinity purified monoclonal antibody (Catalog # P00024-4) at 1:10000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with ChemiDoc MP system. A specific band was detected for Phospho-AKT1 (S124) at approximately 60 kDa. The expected band size for Phospho-AKT1 (S124) is at 60 kDa.
Specific Publications For Anti-Phospho-AKT1 (S124) Rabbit Monoclonal Antibody (P00024-4)
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Customer Reviews
Have you used Anti-Phospho-AKT1 (S124) Rabbit Monoclonal Antibody?
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1 Reviews For Anti-Phospho-AKT1 (S124) Rabbit Monoclonal Antibody
The antibody shows a clear target, correct localization, minimal background, and overall good results.
Excellent
| SKU | P00024-4 |
|---|---|
| Application | Western Blot |
| Sample | mouse 4T1 cells |
| Sample Processing Description | normal 4T1 cells, LPS-stimulated 4T1 cells, cells treated with low, medium, or high doses of the drug, and cells treated with a positive control drug. |
| Other Reagents | RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer |
| Primary Antibody | Anti-AKT1,2,3 Antibody Picoband® |
| Primary Incubation | 1:10000, overnight at 4 ℃ |
| Secondary Antibody | HRP Goat Anti-Rabbit IgG |
| Secondary Incubation | 1:10000, 1 hour in room temperature |
| Detection | Substrate: ECL, Imaging system:ChemiDoc MP |
| Results Summary | Under equal loading conditions, the total AKT protein levels were similar across all groups, while the amount of phosphorylated AKT1 showed significant changes. The blank group had the lowest level, the model group had the highest, and in the drug-treated groups, the levels decreased progressively from low to medium to high dose. The positive control drug group was close to the high-dose group. |
Bolan Guan, Harbin Medical University
Verified customer
Submitted 2025-12-01
Customer Q&As
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4 Customer Q&As for Anti-Phospho-AKT1 (S124) Rabbit Monoclonal Antibody
Question
We have been able to see staining in human cervix carcinoma. What should we do? Is anti-Phospho-AKT1 (S124) Rabbit Monoclonal antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
From literature cervix carcinoma does express AKT1. From Uniprot.org, AKT1 is expressed in left adrenal gland, adrenal gland, muscle ovary, foreskin, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have AKT1 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Foreskin, Pubmed ID: 1718748
Liver, Pubmed ID: 24275569
Muscle, and Ovary, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-07-11
Question
Our team were happy with the WB result of your anti-Phospho-AKT1 (S124) Rabbit Monoclonal antibody. However we have been able to see positive staining in left adrenal gland cytoplasm. using this antibody. Is that expected? Could you tell me where is AKT1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-09-27
Answer
Based on literature, left adrenal gland does express AKT1. Generally AKT1 expresses in cytoplasm. Regarding which tissues have AKT1 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Foreskin, Pubmed ID: 1718748
Liver, Pubmed ID: 24275569
Muscle, and Ovary, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-09-27
Question
We have tried in the past anti-Phospho-AKT1 (S124) Rabbit Monoclonal antibody for WB on foreskin in a previous experiment. I am using mouse, and We want to use the antibody for IP next. I am looking for examining foreskin as well as left adrenal gland in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
Verified Customer
Verified customer
Asked: 2018-01-26
Answer
I have checked the website and datasheets of our anti-Phospho-AKT1 (S124) Rabbit Monoclonal antibody and I see that P00024-4 has been tested on mouse in both WB and IP. Thus P00024-4 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-01-26
Question
We are currently using anti-Phospho-AKT1 (S124) Rabbit Monoclonal antibody P00024-4 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
P. Krishna
Verified customer
Asked: 2014-04-18
Answer
The anti-Phospho-AKT1 (S124) Rabbit Monoclonal antibody (P00024-4) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-04-18
