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AKT1/2/3 antibody (M00024-5) performs well in Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 15 Publications

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Immunofluorescent analysis of MCF-7 cells, using Akt(pan)1/2/3 Antibody.

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Anti-Akt(pan) 1/2/3 Rabbit Monoclonal Antibody

AKT1/2/3 antibody

15 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	Flow Cytometry, IP, IF, IHC, ICC, WB

Tested Applications

AKT1/2/3 antibody was positively detected by IHC in:

Human pituitary tumor tissue, Human astrocytoma tissue, Human ovarian cancer tissue, human testis tissue

Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

AKT1/2/3 antibody was positively detected by ICC/IF in:

MCF-7 cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Recommend

Tested

Recommend

Mouse

Recommend

Rat

Recommend

Product Information

Product Name

Anti-Akt(pan) 1/2/3 Rabbit Monoclonal Antibody
View all AKT1/2/3 Antibodies

Form

Liquid

Uniprot

P31749/P31751/Q9Y243

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

17A20

Isotype

IgG

Immunogen

A synthesized peptide derived from human AKT1/2/3

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

65 kDa

Cite This Product

Anti-Akt(pan) 1/2/3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00024-5)

Protocols

Immunohistochemistry (IHC) protocol for AKT1/2/3 antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for AKT1/2/3 antibody

Review Protocol

Publications

Identification of PRRG1 as a possible molecular target of pancreatic cancer

Published: 2026-05-10

Journal: Cell Death & Disease

Application: WB

Impact Factor: 12.20

Species: Human

PMID: 42108282

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Free Secondary Antibody

Anti-Akt(pan) 1/2/3 Rabbit Monoclonal Antibody

AKT1/2/3 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Identification of PRRG1 as a possible molecular target of pancreatic cancer

β-Ecdysterone Attenuates Ang II–Induced Senescence in Human Aortic Smooth Muscle Cells via Autophagy Activation and ROS Suppression Through AKT/mTOR Pathway Inhibition

Silencing Uracil-DNA glycosylase inhibits colorectal cancer progression

Gu-Jin-Xiao-Ji Decoction potentiates anti-PD-1 immunotherapy in lung cancer by inhibiting the PI3K/AKT/NF-κB/PD-L1 axis

Repurposing Lamivudine: A novel therapeutic strategy for ischemic stroke targeting the glycolytic enzyme Pgk1

Sappanone A attenuates rheumatoid arthritis via inhibiting PI3K/AKT/NF-κB and JAK2/STAT3 signaling pathways in vivo and in vitro

Sinomenine Alleviates Rheumatoid Arthritis by Suppressing the PI3K-Akt Signaling Pathway, as Demonstrated Through Network Pharmacology, Molecular Docking, and Experimental Validation

Distinct roles of TREM2 in central nervous system cancers and peripheral cancers

Pungent agents derived from the fruits of Zanthoxylum armatum DC. are beneficial for ameliorating type 2 diabetes mellitus via regulation of AMPK/PI3K/Akt signaling

Tanshinone IIA Regulates Synaptic Plasticity in Mg2+-Free-Induced Epileptic Hippocampal Neurons via the PI3K/Akt Signaling Pathway

Transcriptome, network analysis, and molecular docking approaches to elucidate the modes of action of Astragaloside IV against myocardial infarction with mitochondrial energy metabolism network-targeted regulation

Effects and mechanisms of substance P on the proliferation and angiogenic differentiation of bone marrow mesenchymal stem cells: Bioinformatics and in vitro experiments

Curcumin-enhanced antitumor effects of sorafenib via regulating the metabolism and tumor microenvironment

Fucoxanthin Alleviates Oxidative Stress through Akt/Sirt1/FoxO3α Signaling to Inhibit HG-Induced Renal Fibrosis in GMCs

Hydrogen sulfide inhibits PCSK9 expression through the PI3K/Akt‑SREBP‑2 signaling pathway to influence lipid metabolism in HepG2 cells

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