Product Info Summary
SKU: | M00024-5 |
---|---|
Size: | 100 μl/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Akt(pan) 1/2/3 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00024-5
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-Akt(pan) 1/2/3 Rabbit Monoclonal Antibody catalog # M00024-5. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Akt(pan) 1/2/3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00024-5)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
17A20
Isotype
IgG
Immunogen
A synthesized peptide derived from human AKT1/2/3
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00024-5 is reactive to in Human, Mouse, Rat
Applications
M00024-5 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
56 kDa
Calculated molecular weight
88068 MW
Background of
The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990; Hollmann and Heinemann, 1994). The GluR1 subunit is widely expressed throughout the nervous system. Phosphorylation of Ser-845 on GluR1 is thought to be mediated by PKA and phosphorylation of this site increases the conductance of the AMPAR (Roche et al., 1996; Banke et al., 2000). In addition, phosphorylation of this site has been linked to synaptic plasticity as well as learning and memory (Lee at al., 2003; Esteban at al., 2003).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Click image to see more details
Western blot analysis of Akt(pan)1/2/3 expression in (1) A549 cell lysate; (2) Mouse brain lysate; (3) Rat brain lysate; (4) Rat heart lysate; (5) Rat kidney lysate.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human pituitary tumor, using the Antibody at 1:250 dilution.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human astrocytoma, using the Antibody at 1:250 dilution.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer, using the Antibody at 1:1000 dilution.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human testis, using Akt(pan)1/2/3 Antibody.
Click image to see more details
Immunofluorescent analysis of MCF-7 cells, using Akt(pan)1/2/3 Antibody.
Click image to see more details
Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:3K dilution)
Specific Publications For Anti-Akt(pan) 1/2/3 Rabbit Monoclonal Antibody (M00024-5)
Hello CJ!
No publications found for M00024-5
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Akt(pan) 1/2/3 Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-Akt(pan) 1/2/3 Rabbit Monoclonal Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question