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Product Info Summary
| SKU: | PB9981 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-ALDH1A2 Antibody Picoband®
SKU/Catalog Number
PB9981
PB1032 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ALDH1A2 Antibody Picoband® catalog # PB9981. Tested in ICC/IF, WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ALDH1A2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9981)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ALDH1A2 recombinant protein (Position: M1-A110). Human ALDH1A2 shares 95.5% amino acid (aa) sequence identity with both mouse and rat ALDH1A2.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9981 is reactive to ALDH1A2 in Human, Monkey
Observed Molecular Weight
57 kDa
Calculated molecular weight
56.7 kDa
Background of ALDH1A2
Aldehyde dehydrogenase 1 family, member A2, also known as ALDH1A2 or retinaldehyde dehydrogenase 2 (RALDH2), is an enzyme that in humans is encoded by the ALDH1A2 gene. This protein belongs to the aldehyde dehydrogenase family of proteins. The product of this gene is an enzyme that catalyzes the synthesis of retinoic acid (RA) from retinaldehyde. Retinoic acid, the active derivative of vitamin A (retinol), is a hormonal signaling molecule that functions in developing and adult tissues. The studies of a similar mouse gene suggest that this enzyme and the cytochrome CYP26A1, concurrently establish local embryonic retinoic acid levels which facilitate posterior organ development and prevent spina bifida. Four transcript variants encoding distinct isoforms have been identified for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9981 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Monkey
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Positive Control
WB: human K562 whole cell, human 293T whole cell, human HepG2 whole cell, monkey kidney tissue, human A549 whole cell
ICC/IF: PC-3 cell
Validation Images & Assay Conditions
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Western blot analysis of ALDH1A2 using anti-ALDH1A2 antibody (PB9981).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: monkey kidney tissue lysates,
Lane 4: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH1A2 antigen affinity purified polyclonal antibody (Catalog # PB9981) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDH1A2 at approximately 57 kDa. The expected band size for ALDH1A2 is at 57 kDa.
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IF analysis of ALDH1A2 using anti-ALDH1A2 antibody (PB9981).
ALDH1A2 was detected in immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-ALDH1A2 Antibody (PB9981) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Specific Publications For Anti-ALDH1A2 Antibody Picoband® (PB9981)
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Customer Q&As
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3 Customer Q&As for Anti-ALDH1A2 Antibody Picoband®
Question
Does anti-ALDH1A2 antibody PB9981 work for WB with testis uterus?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
According to the expression profile of testis uterus, ALDH1A2 is highly expressed in testis uterus. So, it is likely that anti-ALDH1A2 antibody PB9981 will work for WB with testis uterus.
Boster Scientific Support
Answered: 2020-01-24
Question
Is this PB9981 anti-ALDH1A2 antibody reactive to the isotypes of ALDH1A2?
Verified Customer
Verified customer
Asked: 2019-02-05
Answer
The immunogen of PB9981 anti-ALDH1A2 antibody is E.coli-derived human ALDH1A2 recombinant protein (Position: M1-A110). Human ALDH1A2 shares 95.5% amino acid (aa) sequence identity with both mouse and rat ALDH1A2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-02-05
Question
We are currently using anti-ALDH1A2 antibody PB9981 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
T. Walker
Verified customer
Asked: 2016-07-26
Answer
The anti-ALDH1A2 antibody (PB9981) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-07-26
