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- Table of Contents
ALDH2 antibody (PB9472) performs well in Flow Cytometry, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 5 Publications
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Flow Cytometry analysis of HepG2 cells using anti-ALDH2 antibody (PB9472).
Overlay histogram showing HepG2 cells stained with PB9472 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALDH2 Antibody (PB9472, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing HepG2 cells stained with PB9472 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALDH2 Antibody (PB9472, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-ALDH2 Antibody Picoband®
ALDH2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IHC, WB |
Tested Applications
ALDH2 antibody was positively detected by WB in:
human HepG2 whole cell, rat liver tissue, rat lung tissue, rat kidney tissue, mouse kidney tissue, mouse ANA-1 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ALDH2 antibody was positively detected by IHC in:
human liver cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
ALDH2 antibody was positively detected by FC in:
HepG2 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human
Flow Cytometry(Fixed)
1-3 μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Mouse
Tested
Rabbit
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-ALDH2 Antibody Picoband®
View all ALDH2 Antibodies
View all ALDH2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human ALDH2, different from the related mouse sequence by two amino acids, and from the related rat sequence by one amino acid.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
56 kDa
Calculated Molecular Weight
56.4 kDa
Background of ALDH2
ALDH2 (Aldehyde Dehydrogenase 2 Family) is a human gene. The enzyme encoded by this gene belongs to the aldehyde dehydrogenase family of enzymes that catalyze the chemical transformation from acetaldehyde to acetic acid. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Hsu et al. (1985) assigned the ALDH2 locus to chromosome 12 by means of a cDNA probe and Southern blot analysis of somatic cell hybrids. Using an unbiased proteomic search, Chen et al. (2008) identified mitochondrial ALDH2 as an enzyme whose activation correlated with reduced ischemic heart damage in rodent models. A high-throughput screen identified a small molecule activator of ALDH2, which they called Alda-1, that, when administered to rats before an ischemic event, reduced infarct size by 60%, most likely through its inhibitory effect on the formation of cytotoxic aldehydes.
Cite This Product
Anti-ALDH2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9472)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Rat
97%
High Homology
Rabbit
97%
High Homology
Mouse
94%
High Homology
Monkey
94%
High Homology
Sheep
90%
High Homology
Dog
90%
High Homology
Cow
87%
Moderate Homology
Zebrafish
61%
Low homology
Drosophila
35%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ALDH2 antibody
Immunohistochemistry (IHC) protocol for ALDH2 antibody
Flow Cytometry (FC) protocol for ALDH2 antibody
Publications
Application:
Species:
Integrating network pharmacology and bioinformatics to explore and experimentally verify the regulatory effect of Buyang Huanwu decoction on glycolysis and angiogenesis after cerebral infarction
Published: 2023-10-06
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: IF,WB
Impact Factor: 5.40
Species: Mouse
PMID: 37806535
Six Types of Tea Reduce Acute Alcoholism in Mice by Enhancing Ethanol Metabolism, Suppressing Oxidative Stress and Inflammation
Published: 2022-05-23
Journal: Frontiers in Nutrition
Application: WB
Impact Factor: 6.59
Species: Mouse
PMID: 35677547
Bioinformatic and biochemical findings disclosed anti-hepatic steatosis mechanism of calycosin
Published: 2020-05-11
Journal: BIOORGANIC CHEMISTRY
Application: IHC
Impact Factor: 4.83
Species: Mouse
PMID: 32417523
Trichloroethylene injures rat liver and elevates the level of peroxisomal bifunctional enzyme (Ehhadh)
Published: 2020-04-02
Journal: Molecular & Cellular Toxicology
Application: WB
Impact Factor: 1.66
Species: Rat
Disulfiram/Copper Induces Antitumor Activity against Both Nasopharyngeal Cancer Cells and Cancer-Associated Fibroblasts through ROS/MAPK and Ferroptosis Pathways
Published: 2020-01-06
Journal: Cancers
Application: WB
Impact Factor: 6.13
Species: Mouse
PMID: 31935835
Review
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Submit A Review1 Review For Anti-ALDH2 Antibody Picoband®
The antibody was used to perform WB quantitative detection of ALDH2 protein in mouse hippocampus. The experimental results showed clear and distinct bands, indicating that it is suitable for WB detection of ALDH2 protein in mouse tissues.
| SKU | PB9472 |
|---|---|
| Application | Western Blot |
| Sample | Mouse hippocampus tissue |
| Sample Processing Description | The mouse hippocampus tissue was lysed using RIPA lysis buffer supplemented with a protease inhibitor cocktail. Protein concentration was determined, and samples were mixed with 5× protein loading buffer and denatured by heating at 100°C for 10 minutes. Five microliters of each protein sample were loaded per lane onto SDS-PAGE. |
| Primary Antibody | Anti-ALDH2 Antibody Picoband® |
| Primary Incubation | overnight at 4 ℃ |
| Secondary Antibody | HRP-conjugated Anti-Rabbit IgG Secondary Antibody |
| Secondary Incubation | 1 hour in room temperature |
| Detection | Substrate: Ultra-sensitive ECL luminescent reagent (Cat# AR1191), Imaging system: ChemiDoc MP (Bio-Rad) |
| Results Summary | The antibody was used for quantitative WB detection of ALDH2 protein in mouse hippocampus. The results showed clear and distinct bands, indicating its suitability for WB detection of ALDH2 protein in mouse tissues. |
Changbin Yuan, LNUTCM
Verified customer
Submitted 2025-11-06
Product Promise
We promise Anti-ALDH2 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9472
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Sep 19, 2026