Anti-ALDH3A1 Antibody Picoband™

Product Name

Anti-ALDH3A1 Antibody Picoband™

View all Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibodies

SKU/Catalog Number

A01121-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ALDH3A1 Antibody Picoband™ catalog # A01121-3. Tested in IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-ALDH3A1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01121-3)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human ALDH3A1, identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01121-3 is reactive to ALDH3A1 in Human, Mouse, Rat

Applications

A01121-3 is guaranteed for IF, ICC, WB Boster Guarantee

Observed Molecular Weight

55 kDa

Calculated molecular weight

50.395kDa

Background of Aldehyde Dehydrogenase 3-A1/ALDH3A1

ALDH3A1(Aldehyde Dehydrogenase, Family 3, Subfamily A, Member 1), also known as ALDH3, is an enzyme that in humans is encoded by the ALDH3A1 gene. By in situ hybridization, Hiraoka et al.(1995) mapped the ALDH3 gene to 17p11.2. ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. This protein preferentially oxidizes aromatic aldehyde substrates. It may play a role in the oxidation of toxic aldehydes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of ALDH3A1 using anti-ALDH3A1 antibody (A01121-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: rat stomach tissue lysates,
Lane 4: rat lung tissue lysates,
Lane 5: mouse stomach tissue lysates,
Lane 6: mouse lung tissue lysates,
Lane 7: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH3A1 antigen affinity purified polyclonal antibody (Catalog # A01121-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDH3A1 at approximately 55 kDa. The expected band size for ALDH3A1 is at 55 kDa.

Click image to see more details

Figure 2. IF analysis of ALDH3A1 using anti-ALDH3A1 antibody (A01121-3).
ALDH3A1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ALDH3A1 Antibody (A01121-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For ALDH3A1 (Source: Uniprot.org, NCBI)

Alternative Names

aldehyde dehydrogenase 3 family, member A1; Aldehyde dehydrogenase 3; Aldehyde Dehydrogenase 3A1; Aldehyde Dehydrogenase 3-A1; Aldehyde dehydrogenase family 3 member A1; aldehyde dehydrogenase isozyme 3; aldehyde dehydrogenase type III; ALDH3A1; ALDH3aldehyde dehydrogenase, dimeric NADP-preferring; ALDHIII; EC 1.2.1; EC 1.2.1.5; MGC10406; stomach aldehyde dehydrogenase ALDH3A1 ALDH3, ALDHIII aldehyde dehydrogenase 3 family member A1 aldehyde dehydrogenase, dimeric NADP-preferring|aldehyde dehydrogenase isozyme 3|aldehyde dehydrogenase type III|stomach aldehyde dehydrogenase

For more info on ALDH3A1, check out the ALDH3A1 Infographic

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In this infographic, you will see the following information for ALDH3A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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