Anti-ALIX Rabbit Monoclonal Antibody

Product Name

Anti-ALIX Rabbit Monoclonal Antibody

View all Alix Antibodies

SKU/Catalog Number

M01751

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-ALIX Rabbit Monoclonal Antibody catalog # M01751. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ALIX Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01751)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AEBO-16

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human ALIX

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01751 is reactive to PDCD6IP in Human, Mouse, Rat

Applications

M01751 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

96 kDa

Calculated molecular weight

96.023kDa

Background of Alix

Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
FC 1:100

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of ALIX using anti-ALIX antibody (M01751).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human Raji whole cell lysates,
Lane 3: human Daudi whole cell lysates,
Lane 4: rat liver tissue lysates,
Lane 5: rat lung tissue lysates,
Lane 6: mouse liver tissue lysates,
Lane 7: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALIX antigen affinity purified polyclonal antibody (Catalog # M01751) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALIX at approximately 96 kDa. The expected band size for ALIX is at 96 kDa.

Gene/Protein Information For PDCD6IP (Source: Uniprot.org, NCBI)

Alternative Names

AIP1DRIP4; ALG-2 interacting protein 1; ALG-2 interacting protein X; ALG-2-interacting protein 1; alinx; Alix; apoptosis-linked gene 2-interacting protein X; dopamine receptor interacting protein 4; HP95; KIAA1375; MGC17003; PDCD6-interacting protein; programmed cell death 6 interacting protein; programmed cell death 6-interacting protein PDCD6IP AIP1, ALIX, DRIP4, HP95 programmed cell death 6 interacting protein programmed cell death 6-interacting protein|ALG-2 interacting protein 1|ALG-2-interacting protein X|PDCD6-interacting protein|apoptosis-linked gene 2-interacting protein X|dopamine receptor interacting protein 4

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In this infographic, you will see the following information for PDCD6IP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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